Optimum conditions for extracting collagen from the tunica albuginea of immunologically castrated pig testes and the functional properties of the isolated collagen

被引：18

作者：

Simoes, Gislaine Silveira ^{[1
]}

Facco Silveira, Expedito Tadeu ^{[2
]}

de Oliveira, Simone Raymundo ^{[2
]}

Poleze, Evandro ^{[4
]}

Allison, Jim R. D. ^{[5
]}

Ida, Elza Iouko ^{[1
]}

Shimokomaki, Massami ^{[1
,3
]}

机构：

[1] Univ Estadual Londrina, Dept Food Sci & Technol, BR-86057970 Londrina, Parana, Brazil

[2] Meat Technol Ctr, Inst Food Technol, BR-13070178 Sao Paulo, Brazil

[3] Fed Technol Univ Parana, BR-86036370 Londrina, Parana, Brazil

[4] Pfizer Anim Hlth, Guarulhos, Brazil

[5] Pfizer Anim Hlth, Florham Pk, NJ USA

来源：

MEAT SCIENCE | 2014年 / 96卷 / 04期

关键词：

Factorial design; Central composite rotatable design; Hydroxyproline; Collagen emulsified products; ACID-SOLUBLE COLLAGEN; AGE-RELATED-CHANGES; COD GADUS-MORHUA; CHICKEN FEET; I COLLAGEN; SKIN; MEAT; PEPSIN; HYDROXYPROLINE; OPTIMIZATION;

D O I：

10.1016/j.meatsci.2013.10.038

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

This study evaluated alternative methods for extracting collagen from the tunica albuginea of pig testes and characterized the functional properties of the isolated collagen. Using the statistical tools of factorial design (2(4-1)) and a central composite rotatable design (2(3)), it was concluded that the best conditions were 0.83 mol L-1 acetic acid, 0.24% pepsin and 28 h of hydrolysis to isolate 82.54 g of collagen per 100 g of sample. This purified collagen had improved functional properties in relation to bovine skin collagen, including water solubility, water-holding capacity, emulsifying capacity and emulsion stability. These results suggest that isolated collagen from the tunica albuginea can be used in pharmaceutical and food products. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：1460 / 1468

页数：9

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