Fabrication of silver nanoparticles by Phoma glomerata and its combined effect against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus

被引：287

作者：

Birla, S. S. ^{[1
]}

Tiwari, V. V. ^{[1
]}

Gade, A. K. ^{[1
]}

Ingle, A. P. ^{[1
]}

Yadav, A. P. ^{[1
]}

Rai, M. K. ^{[1
]}

机构：

[1] SGB Amravati Univ, Dept Biotechnol, Amravati 444602, Maharashtra, India

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2009年 / 48卷 / 02期

关键词：

drug resistance; extracellular; Fourier transform infrared spectroscopy; Phoma glomerata; scanning electron microscopy; silver nanoparticles; EXTRACELLULAR BIOSYNTHESIS; MICROBIAL SYNTHESIS; GOLD NANOPARTICLES; FUNGUS; CRYSTALLINE; BACTERIA; IONS;

D O I：

10.1111/j.1472-765X.2008.02510.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report extracellular synthesis of silver nanoparticles (Ag-NPs) from Phoma glomerata and its efficacy against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. The bacteria exhibiting resistance to various antibiotics showed remarkable sensitivity, when used in combination of antibiotics and Ag-NPs. Biosynthesis of Ag-NPs was carried out by challenging the fungal cell filtrate with 1 mmol l(-1) silver nitrate. The Ag-NPs were characterized with the help of UV-Visible spectrophotometer and Fourier transform infrared spectroscopy. Scanning electron microscopy was carried out to detect the size of Ag-NPs. Evaluation of the combined effect(s) was studied by disc diffusion method against E. coli, Staph. aureus and Ps. aeruginosa. The biosynthesis route seems to be eco-friendly and easy to scale up the process. Thus, these Ag-NPs may prove as a better candidate for drugs and can potentially eliminate the problem of chemical agents because of their biogenic nature. The bacterial resistance against antibiotics has been increasing with alarming rate. To overcome this problem, there is a pressing need to develop bactericidal agents. Ag-NPs may prove to be an answer to drug-resistant bacteria.

引用

页码：173 / 179

页数：7

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