Chronic feeding of a low boron diet adversely affects reproduction and development in Xenopus laevis

被引:60
作者
Fort, DJ [1 ]
Stover, EL
Strong, PL
Murray, FJ
Keen, CL
机构
[1] Stover Grp, R&D Lab Div, Stillwater, OK 74074 USA
[2] Murray & Associates, San Jose, CA 95138 USA
[3] US Borax, Valencia, CA 91355 USA
[4] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA
关键词
development; reproduction; nutritional essentiality; Xenopus laevis;
D O I
10.1093/jn/129.11.2055
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
The aims of this work were as follows: 1) to determine whether a purified diet currently used for studies with rats was acceptable for reproductive studies in frogs; and 2) to determine whether frogs are sensitive to a deficit of boron (B) in the diet. Adult Xenopus laevis were fed a nonpurified beef liver and lung (BLL) diet (310 mu g B/kg), a purified diet supplemented with boron (+B; 1850 mu g B/kg), or a purified diet low in boron (-B; 45 mu g B/kg) for 120 d. Frogs fed the BLL and +B diets produced 11.3 and 12.2% necrotic eggs, respectively. Abnormal gastrulation occurred in <4% of the fertilized eggs in both groups, and 96-h larval survival exceeded 75% in both groups. In contrast, frogs fed the -B diet for 120 d produced a high proportion of necrotic eggs (54%). Fertilized embryos from the -B diet-fed frogs showed a high frequency of abnormal gastrulation (26.8%), and >80% of the embryos died before 96 h of development. Mean embryo cell counts at X. laevis developmental stage 7.5 (mid-blastula) were significantly lower in the -B embryos than in the BLL or +B embryos. BLL and -B embryos grown in low boron culture media had a high frequency of malformations compared with embryos grown in boron-supplemented media. These studies show that a purified diet that has been used in rodent studies was acceptable for reproduction studies in X. laevis. This work also demonstrates that a diet low in boron markedly impairs normal reproductive function in adult X. laevis, and that administration of the low boron diet results in an increase in both incidence and severity of adverse effects. In addition, these studies demonstrate the usefulness of the X. laevis model in nutrition studies.
引用
收藏
页码:2055 / 2060
页数:6
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