Collection of nasal secretions for immunological analysis

被引:71
作者
Watelet, JB [1 ]
Gevaert, P [1 ]
Holtappels, G [1 ]
Van Cauwenberge, P [1 ]
Bachert, C [1 ]
机构
[1] Ghent Univ Hosp, Dept Otorhinolaryngol, Ghent, Belgium
关键词
nasal secretions; sampling; technique;
D O I
10.1007/s00405-003-0691-y
中图分类号
R76 [耳鼻咽喉科学];
学科分类号
100213 ;
摘要
The biochemical analysis of nasal secretions has become essential in the study of nasal or sinus diseases and the monitoring of medical and surgical treatment. The nasal fluid greatly reflects the inflammatory activity within the nasal mucosa. This paper discusses techniques for nasal fluid collection described before and proposes a new approach for the collection and calculation of nasal secretions based on sinus packs. The method is non-invasive, well standardized and reproducible and therefore may serve as a valid tool for future investigations.
引用
收藏
页码:242 / 246
页数:5
相关论文
共 25 条
[1]   Elevated levels of myeloperoxidase, pro-inflammatory cytokines and chemokines in naturally acquired upper respiratory tract infections [J].
Bachert, C ;
van Kempen, MJP ;
Höpken, K ;
Holtappels, G ;
Wagenmann, M .
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY, 2001, 258 (08) :406-412
[2]   PHYSIOLOGICAL-RESPONSES AND HISTAMINE-RELEASE AFTER NASAL ANTIGEN CHALLENGE - EFFECT OF ATROPINE [J].
BAROODY, FM ;
FORD, S ;
LICHTENSTEIN, LM ;
KAGEYSOBOTKA, A ;
NACLERIO, RM .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1994, 149 (06) :1457-1465
[3]   NASAL SECRETIONS FROM PATIENTS WITH POLYPS AND HEALTHY-INDIVIDUALS, COLLECTED WITH A NEW ASPIRATION SYSTEM - EVALUATION OF TOTAL PROTEIN AND IMMUNOGLOBULIN CONCENTRATIONS [J].
BIEWENGA, J ;
STOOP, AE ;
BAKER, HE ;
SWART, SJ ;
NAUTA, JJP ;
VANKAMP, GJ ;
VANDERBAAN, S .
ANNALS OF CLINICAL BIOCHEMISTRY, 1991, 28 :260-266
[4]  
Gilain L, 2000, Ann Otolaryngol Chir Cervicofac, V117, P91
[5]   AMINO-ACID COMPOSITION OF HUMAN NASAL MUCUS - NORMAL SUBJECTS [J].
HILDING, AC ;
COWLES, EJ ;
STUART, JH .
ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY, 1973, 82 (01) :75-79
[6]   Nasal biopsy is superior to nasal smear for finding eosinophils in nonallergic rhinitis [J].
Ingels, K ;
Durdurez, JP ;
Cuvelier, C ;
vanCauwenberge, P .
ALLERGY, 1997, 52 (03) :338-341
[7]  
Jankowski R, 1992, Ann Otolaryngol Chir Cervicofac, V109, P39
[8]  
JEFFERY PK, 1983, AM REV RESPIR DIS, V128, pS14
[9]   Levels of intracellular protein and messenger RNA of mucin and lysozyme in normal human nasal and polyp epithelium [J].
Kim, SS ;
Kim, KS ;
Lee, JG ;
Park, IY ;
Koo, JS ;
Yoon, JH .
LARYNGOSCOPE, 2000, 110 (02) :276-280
[10]   Immunoglobulins in nasal secretions of healthy humans: Structural integrity of secretory immunoglobulin A1 (IgA1) and occurrence of neutralizing antibodies to IgA1 proteases of nasal bacteria [J].
Kirkeby, L ;
Rasmussen, TT ;
Reinholdt, J ;
Kilian, M .
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2000, 7 (01) :31-39