Production and characterisation of a biosurfactant isolated from Pseudomonas aeruginosa UW-1

被引:181
作者
Sim, L [1 ]
Ward, OP [1 ]
Li, ZY [1 ]
机构
[1] UNIV WATERLOO,DEPT BIOL,MICROBIAL BIOTECHNOL LAB,WATERLOO,ON N2L 3G1,CANADA
来源
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 1997年 / 19卷 / 04期
关键词
Pseudomanas aeruginosa; rhamnolipid; biosurfactant; fermentation; purification;
D O I
10.1038/sj.jim.2900450
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Pseudomonas aeruginosa UW-1 produced 17-24 g L-1 rhamnolipid in vegetable oil-containing media in shake flask cultures in 13 days, In time course studies of growth and rhamnolipid production in a salts medium containing 6% canola ail, total bacterial count reached 2.6 x 10(10) CFU ml(-1) after 48 h and a maximum rhamnolipid yield of 24.3 g L-1 was obtained after 9 days, Rhamnolipid components were purified and separated by chloroform-methanol extraction and TLC chromatography. The major rhamnolipid components were characterised as L-rhamnosyl-beta-hydroxydecanoyl-beta-hydroxydecanoate and L-rhamnosyl-L-rhamnosyl-beta-hydroxydecanoyl-beta-hydroxydecanoate by nuclear magnetic resonance and mass spectrometry, The components were separated preparatively by silica gel column chromatography, The recovered monorhamnosyl fraction contained no dirhamnosyl moiety while the recovered dirhamnosyl fraction contained 5% of the monorhamnosyl moiety when analyzed by HPLC. The ratio of mono-to dirhamnosyl components produced by P, aeruginosa UW-1 was determined by HPLC to be 4:1 by weight, Purified mono-and dirhamnosyl components had the same CMC value of 40 mu g ml(-1) and decreased the surface tension of water to 27.7 and 30.4 dynes cm(-1), respectively.
引用
收藏
页码:232 / 238
页数:7
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