Detection of All Four Human Metapneumovirus Subtypes in Nasopharyngeal Specimens from Children with Respiratory Disease in Uberlandia, Brazil

被引:17
作者
Carneiro, Bruno M. [1 ]
Yokosawa, Jonny [1 ]
Arbiza, Juan [2 ]
Costa, Lourenco F. [1 ]
Mirazo, Santiago [2 ]
Nepomuceno, Lysa L. [1 ]
Oliveira, Thelma F. M. [1 ]
Goulart, Luiz Ricardo [3 ]
Vieira, Carlos U. [3 ]
Freitas, Guilherme R. O. [1 ]
Paula, Nayhanne T. [1 ]
Queiroz, Divina A. O. [1 ]
机构
[1] Univ Fed Uberlandia, Virol Lab, Inst Ciencias Biomed, BR-38400782 Uberlandia, MG, Brazil
[2] Univ Republica, Fac Ciencias, Secc Virol, Montevideo, Uruguay
[3] Univ Fed Uberlandia, Inst Genet & Bioquim, Lab Nanobiotecnol, BR-38400782 Uberlandia, MG, Brazil
关键词
respiratory virus diseases; human metapneumovirus; infants and young children; SYNCYTIAL VIRUS; CIRCULATION PATTERNS; PEDIATRIC-PATIENTS; YOUNG-CHILDREN; GROUP-A; INFECTION; PREVALENCE; GENOTYPE; VARIABILITY; EPIDEMICS;
D O I
10.1002/jmv.21555
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The human metapneumovirus (hMPV) is a pathogen of the respiratory tract identified first in the Netherlands in 2001 and since then it has been detected worldwide. The purpose of this study was to identify and characterize hMPV in samples collected from children <5 years presenting with acute respiratory disease (ARD) seen at a public hospital in Uberlandia, in Southeastern Brazil. One hundred fourteen nasopharyngeal aspirates (NPAs) samples that were negative for the presence of nine other respiratory viruses were tested by reverse transcription polymerase chain reaction (RT-PCR) for the presence of hMPV RNA. Fourteen out of 114 (12.3%) samples were positive for presence of hMPV RNA. PCR products, obtained by the amplification of partial nucleotide sequence of gene N, were sequenced and compared with sequences deposited in GenBank. Sequences from eight samples were obtained and all four subtypes were identified. Also, the recently proposed sublineages "a" and "b" of subtype A2 were found; mean age was 21 months old; upper respiratory tract infection (URTI) was the most common clinical symptom; the virus was detected in samples collected from March to November, a period that corresponds to late summer to mid-spring in Brazil. This is the first study to describe the circulation of all hMPV subtypes in Minas Gerais state. J. Med. Virol. 81: 1814-1818, 2009. (C) 2009 Wiley-Liss, Inc.
引用
收藏
页码:1814 / 1818
页数:5
相关论文
共 30 条
[1]   Genetic variability of human metapneumovirus infection: Evidence of a shift in viral genotype without a change in illness [J].
Agapov, E ;
Sumino, KC ;
Gaudreault-Keener, M ;
Storch, GA ;
Holtzman, MJ .
JOURNAL OF INFECTIOUS DISEASES, 2006, 193 (03) :396-403
[2]   Positive Selection Results in Frequent Reversible Amino Acid Replacements in the G Protein Gene of Human Respiratory Syncytial Virus [J].
Botosso, Viviane F. ;
Zanotto, Paolo M. de A. ;
Ueda, Mirthes ;
Arruda, Eurico ;
Gilio, Alfredo E. ;
Vieira, Sandra E. ;
Stewien, Klaus E. ;
Peret, Teresa C. T. ;
Jamal, Leda F. ;
Pardini, Maria I. de M. C. ;
Pinho, Joao R. R. ;
Massad, Eduardo ;
Sant'Anna, Osvaldo A. ;
Holmes, Eddie C. ;
Durigon, Edison L. .
PLOS PATHOGENS, 2009, 5 (01)
[3]   Genotype variability of human metapneumovirus, South Korea [J].
Chung, Ju-Young ;
Han, Tae Hee ;
Kim, Sang Woo ;
Hwang, Eung-Soo .
JOURNAL OF MEDICAL VIROLOGY, 2008, 80 (05) :902-905
[4]   Respiratory viruses in children younger than five years old with acute respiratory disease from 2001 to 2004 in Uberlandia, MG, Brazil [J].
Costa, L. F. ;
Yokosawa, J. ;
Mantese, O. C. ;
Oliveira, T. F. M. ;
Silveira, H. L. ;
Nepomuceno, L. L. ;
Moreira, L. S. ;
Dyonisio, G. ;
Rossi, L. M. G. ;
Oliveira, R. C. ;
Ribeiro, L. Z. G. ;
Queiroz, D. A. O. .
MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2006, 101 (03) :301-306
[5]   Human metapneumovirus and respiratory syncytial virus, Brazil [J].
Cuevas, LE ;
Ben Nasser, AM ;
Dove, W ;
Gurgel, RQ ;
Greensill, J ;
Hart, CA .
EMERGING INFECTIOUS DISEASES, 2003, 9 (12) :1626-1628
[6]   Variant isolates of human metapneumovirus subgroup B genotype 1 in Campinas, Brazil [J].
Da Silva, Luciana Helena Antoniassi ;
Spilki, Fernando Rosado ;
Riccetto, Adriana Gut Lopes ;
de Almeida, Renata Servan ;
Baracat, Emilio Carlos Elias ;
Arns, Clarice Weis .
JOURNAL OF CLINICAL VIROLOGY, 2008, 42 (01) :78-81
[7]   Acute respiratory infection by human metapneumovirus in children in southern Brazil [J].
Debur, Maria do Carmo ;
Bordignon, Juliano ;
Duarte dos Santos, Claudia Nunes ;
Vidal, Luine Rosele ;
Nogueira, Meri Bordignon ;
de Almeida, Sergio Monteiro ;
Raboni, Sonia Mara .
JOURNAL OF CLINICAL VIROLOGY, 2007, 39 (01) :59-62
[8]   Evidence of human metapneumovirus in children in Argentina [J].
Galiano, M ;
Videla, C ;
Puch, SS ;
Martínez, A ;
Echavarría, M ;
Carballal, G .
JOURNAL OF MEDICAL VIROLOGY, 2004, 72 (02) :299-303
[9]  
GARCIA O, 1994, J VIROL, V68, P5448
[10]   Changing circulation rate of human metapneumovirus strains and types among hospitalized pediatric patients during three consecutive winter-spring seasons [J].
Gerna, G ;
Campanini, G ;
Rovida, F ;
Sarasini, A ;
Lilleri, D ;
Paolucci, S ;
Marchi, A ;
Baldanti, F ;
Revello, MG .
ARCHIVES OF VIROLOGY, 2005, 150 (11) :2365-2375