Enterococcus faecalis Biofilms Eradication by Root Canal Irrigants

被引：91

作者：

Teresa Arias-Moliz, Maria ^{[1
]}

Maria Ferrer-Luque, Carmen ^{[2
]}

Espigares-Garcia, Miguel ^{[3
]}

Baca, Pilar ^{[2
]}

机构：

[1] Univ Granada, Dept Microbiol, Sch Dent, E-18071 Granada, Spain

[2] Univ Granada, Sch Dent, Dept Prevent & Operat Dent, E-18071 Granada, Spain

[3] Univ Granada, Dept Prevent Med & Publ Hlth, Sch Pharm, E-18071 Granada, Spain

来源：

JOURNAL OF ENDODONTICS | 2009年 / 35卷 / 05期

关键词：

Biofilm; chlorhexidine; citric acid; Enterococcus faecalis; EDTA; irrigants; phosphoric acid; sodium hypochlorite; VITRO ANTIMICROBIAL ACTIVITY; IN-VITRO; SODIUM-HYPOCHLORITE; MICROBIAL BIOFILMS; BACTERIAL BIOFILMS; DENTIN; SUSCEPTIBILITY; IRRIGATION; CHLORHEXIDINE; MEDICATIONS;

D O I：

10.1016/j.joen.2009.01.018

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

The aim of this study was to evaluate the minimal biofilm eradication concentration (MBEC) of sodium hypechlorite (NaOCl), chlorhexidine (CHX), EDTA, and citric and phosphoric acids after 1, 5, and 10 minutes of exposure to biofilms of Enterococcus faecalis. The biofilms grew in the MBEC high-throughput device for 24 hours at 37 degrees C and were exposed to 10 serial two-fold dilutions of each irrigating solution. The viable cell counts were log(10) transformed, and a concentration of an irrigant was considered to eradicate the biofilms when it produced a reduction of >= 5 logarithmic units. NaOCl was the most effective agent, capable of eradicating the biofilms after 1 minute at a concentration of 0.00625%. CHX eradicated biofilm after 5 minutes at 2%. EDTA and citric and phosphoric acid solutions were not effective against the biofilms at any concentration or time tested. (J Endod 2009;35:711-714)

引用

页码：711 / 714

页数：4

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