Long-term dietary supplementation with L-arginine prevents age-related reduction in renal function

被引:36
作者
Reckelhoff, JF
Kellum, JA
Racusen, LC
Hildebrandt, DA
机构
[1] UNIV MISSISSIPPI, MED CTR, DEPT PHYSIOL, JACKSON, MS 39216 USA
[2] UNIV MISSISSIPPI, MED CTR, DEPT BIOPHYS, JACKSON, MS 39216 USA
[3] UNIV MISSISSIPPI, MED CTR, DEPT CARDIOTHORAC SURG, JACKSON, MS 39216 USA
[4] JOHNS HOPKINS UNIV HOSP, DEPT PATHOL, BALTIMORE, MD 21205 USA
关键词
nitric oxide; glomerular filtration rate; glomerular sclerosis;
D O I
10.1152/ajpregu.1997.272.6.R1768
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Aging is associated with loss of nephron function and reductions in serum L-arginine and excretion of nitric oxide (NO) metabolites. The present study was performed to determine if long-term dietary treatment with L-arginine, the NO synthase substrate, could prevent age-related renal injury. Studies were performed in four groups of rats, aged 12-13 mo, for 8 mo: group 1 received L-arginine (2% in 2.5% corn syrup, n = 5); group 2 received sodium nitrite, an NO donor (0.1%, in 2.5% syrup, n = 7); group 3 was an untreated control group (n = 7); group 4 was treated with 2.5% corn syrup (n = 5). Urinary protein increased and urinary nitrate/nitrite decreased with age in controls, but, during L-arginine treatment, urinary protein decreased and nitrate/nitrite increased. Two weeks after L-arginine was stopped, urinary protein had increased and nitrate/nitrite had decreased to the same level as in controls. L-Arginine treatment increased glomerular filtration rate (GFR) by 50% compared with untreated controls. In contrast, nitrite had no effect on GFR. Morphologically, L-arginine protected against aging injury by reducing the number of sclerotic glomeruli. In summary, we found that L-arginine prevented the age-related glomerular injury and reduction in GFR. The mechanism of protection, however. may be independent of NO.
引用
收藏
页码:R1768 / R1774
页数:7
相关论文
共 32 条
[1]   CHRONIC BLOCKADE OF NITRIC-OXIDE SYNTHESIS IN THE RAT PRODUCES SYSTEMIC HYPERTENSION AND GLOMERULAR DAMAGE [J].
BAYLIS, C ;
MITRUKA, B ;
DENG, A .
JOURNAL OF CLINICAL INVESTIGATION, 1992, 90 (01) :278-281
[2]   L-ARGININE DEPLETION BY ARGINASE REDUCES NITRIC-OXIDE PRODUCTION IN ENDOTOXIC-SHOCK - AN ELECTRON-PARAMAGNETIC-RESONANCE STUDY [J].
BUNE, AJ ;
SHERGILL, JK ;
CAMMACK, R ;
COOK, HT .
FEBS LETTERS, 1995, 366 (2-3) :127-130
[3]   L-ARGININE ABROGATES SALT-SENSITIVE HYPERTENSION IN DAHL RAPP RATS [J].
CHEN, PY ;
SANDERS, PW .
JOURNAL OF CLINICAL INVESTIGATION, 1991, 88 (05) :1559-1567
[4]   ARGININE METABOLISM IN EXPERIMENTAL GLOMERULONEPHRITIS - INTERACTION BETWEEN NITRIC-OXIDE SYNTHASE AND ARGINASE [J].
COOK, HT ;
JANSEN, A ;
LEWIS, S ;
LARGEN, P ;
ODONNELL, M ;
REAVELEY, D ;
CATTELL, V .
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 1994, 267 (04) :F646-F653
[5]   SINGLE-NEPHRON FILTRATION-RATE AND PROXIMAL REABSORPTION IN AGING RATS [J].
CORMAN, B ;
ROINEL, N .
AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 260 (01) :F75-F80
[6]   PROTEINASES AND THE GLOMERULUS - THEIR ROLE IN GLOMERULAR-DISEASES [J].
DAVIES, M ;
COLES, GA ;
THOMAS, GJ ;
MARTIN, J ;
LOVETT, DH .
KLINISCHE WOCHENSCHRIFT, 1990, 68 (22) :1145-1149
[7]   CHRONIC TOXICITY OF SODIUM-NITRITE IN THE MALE F344 RAT [J].
GRANT, D ;
BUTLER, WH .
FOOD AND CHEMICAL TOXICOLOGY, 1989, 27 (09) :565-571
[8]  
Hennington BS, 1996, FASEB J, V10, P221
[9]  
ICHIKAWA I, 1978, Renal Physiology, V1, P121
[10]  
IGNARRO LJ, 1981, J PHARMACOL EXP THER, V218, P739