Receptor activation alters inner surface potential during phagocytosis

被引：255

作者：

Yeung, Tony

Terebiznik, Mauricio

Yu, Liming

Silvius, John

Abidi, Wasif M.

Philips, Mark

Levine, Tim

Kapus, Andras

Grinstein, Sergio

机构：

[1] Hosp Sick Children, Div Cell Biol, Toronto, ON M5G 1X8, Canada

[2] Hosp Sick Children, Gastroenterol Hepatol & Nutr Dept, Toronto, ON M5G 1X8, Canada

[3] Univ Toronto, Inst Med Sci, Toronto, ON M5S 1A8, Canada

[4] McGill Univ, Dept Biochem, Montreal, PQ H3G 1Y6, Canada

[5] NYU, Sch Med, Dept Med, New York, NY 10016 USA

[6] UCL, Div Cell Biol, London EC1V 9EL, England

[7] St Michaels Hosp, Res Inst, Toronto, ON M5B 1W8, Canada

来源：

SCIENCE | 2006年 / 313卷 / 5785期

关键词：

D O I：

10.1126/science.1129551

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The surface potential of biological membranes varies according to their lipid composition. We devised genetically encoded probes to assess surface potential in intact cells. These probes revealed marked, localized alterations in the charge of the inner surface of the plasma membrane of macrophages during the course of phagocytosis. Hydrolysis of phosphoinositides and displacement of phosphatidylserine accounted for the change in surface potential at the phagosomal cup. Signaling molecules such as K-Ras, Rac1, and c-Src that are targeted to the membrane by electrostatic interactions were rapidly released from membrane subdomains where the surface charge was altered by lipid remodeling during phagocytosis.

引用

页码：347 / 351

页数：5

共 9 条

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