Isolation, analysis, and deletion of the gene coding for subunit IV of cytochrome c oxidase in Paracoccus denitrificans

被引：36

作者：

Witt, H ^{[1
]}

Ludwig, B ^{[1
]}

机构：

[1] UNIV FRANKFURT,INST BIOCHEM,D-60439 FRANKFURT,GERMANY

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1997年 / 272卷 / 09期

关键词：

D O I：

10.1074/jbc.272.9.5514

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene coding for subunit IV of the cytochrome c oxidase in Paracoccus denitrificans has been cloned and sequenced. The derived amino acid sequence shows no significant homology to any known protein. Gene deletion has no consequences for the integrity of the complex and its spectral and enzymatic properties. Complementation of the deletion mutant in trans results in expression of subunit IV; sequence analysis of the 5'-noncoding region leads to the identification of a putative promoter sequence.

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页码：5514 / 5517

页数：4

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