Rapid mixing methods for exploring the kinetics of protein folding

被引:54
作者
Roder, H
Maki, K
Cheng, H
Shastry, MCR
机构
[1] Fox Chase Canc Ctr, Div Basic Sci, Philadelphia, PA 19111 USA
[2] Univ Penn, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
关键词
ultrafast mixing; stopped-flow; continuous-flow; fluorescence; NMR;
D O I
10.1016/j.ymeth.2004.03.003
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Information on the time-dependence of molecular species is critical for elucidating reaction mechanisms in chemistry and biology. Rapid flow experiments involving turbulent mixing of two or more solutions continue to be the main source of kinetic information on protein folding and other biochemical processes, such as ligand binding and enzymatic reactions. Recent advances in mixer design and detection methods have opened a new window for exploring conformational changes in proteins on the microsecond time scale. These developments have been especially important for exploring early stages of protein folding. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:15 / 27
页数:13
相关论文
共 87 条
[1]   Conformational landscape of cytochrome c folding studied by microsecond-resolved small-angle x-ray scattering [J].
Akiyama, S ;
Takahashi, S ;
Kimura, T ;
Ishimori, K ;
Morishima, I ;
Nishikawa, Y ;
Fujisawa, T .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (03) :1329-1334
[2]  
Akiyama S, 2000, NAT STRUCT BIOL, V7, P514
[3]   PRIMARY STRUCTURE EFFECTS ON PEPTIDE GROUP HYDROGEN-EXCHANGE [J].
BAI, YW ;
MILNE, JS ;
MAYNE, L ;
ENGLANDER, SW .
PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1993, 17 (01) :75-86
[4]   FOLLOWING PROTEIN-FOLDING IN REAL-TIME USING NMR-SPECTROSCOPY [J].
BALBACH, J ;
FORGE, V ;
VANNULAND, NAJ ;
WINDER, SL ;
HORE, PJ ;
DOBSON, CM .
NATURE STRUCTURAL BIOLOGY, 1995, 2 (10) :865-870
[5]   Is protein folding hierarchic? II. Folding intermediates and transition states [J].
Baldwin, RL ;
Rose, GD .
TRENDS IN BIOCHEMICAL SCIENCES, 1999, 24 (02) :77-83
[6]  
BEECHEM JM, 1990, SPIE P, V1204, P686
[7]   HIGH RESOLUTION MIXER FOR STUDY OF KINETICS OF RAPID REACTIONS IN SOLUTION [J].
BERGER, RL ;
BALKO, B ;
CHAPMAN, HF .
REVIEW OF SCIENTIFIC INSTRUMENTS, 1968, 39 (04) :493-&
[8]   DETERMINATION OF THE DEAD TIME OF A STOPPED-FLOW FLUOROMETER [J].
BRISSETTE, P ;
BALLOU, DP ;
MASSEY, V .
ANALYTICAL BIOCHEMISTRY, 1989, 181 (02) :234-238
[9]   Fast events in protein folding: The time evolution of primary processes [J].
Callender, RH ;
Dyer, RB ;
Gilmanshin, R ;
Woodruff, WH .
ANNUAL REVIEW OF PHYSICAL CHEMISTRY, 1998, 49 :173-202
[10]  
Capaldi AP, 2001, NAT STRUCT BIOL, V8, P68