Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection

被引:76
作者
Klejdus, B
Vacek, J
Adam, V
Zehnálek, J
Kizek, R
Trnková, L
Kubán, V
机构
[1] Mendel Univ Brno, Dept Chem & Biochem, CZ-61300 Brno, Czech Republic
[2] Masaryk Univ, Fac Sci, Dept Theoret & Phys Chem, CZ-61137 Brno, Czech Republic
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2004年 / 806卷 / 02期
关键词
isoflavones;
D O I
10.1016/j.jchromb.2004.03.044
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A highly sensitive high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) was developed for the determination of isoflavones. Electrochemical behaviour of daidzein and genistein was studied on carbon paste electrode (CPE) by adsorptive transfer stripping square wave voltammetry. The obtained electrochemical results were used for the development of HPLC-ED method. Furthermore, isoflavones were separated on an Atlantis dC18 column using a mobile phase consisting of acetonitrile (solvent A) and 0.15 M acetate buffer of pH 5.5 (solvent B) at a flow rate 0.4 mL/min. A linear gradient profile (solvent B) was at 0-2 min 87%; 22 min 60%; 27 min 50%: 31 min 45%; 47 min 87%. Full scan of multi-channel coulometric detection was tested and optimal potential at 450 mV was chosen for our purposes. Calibration curves were linear (daidzein R-2 = 0.9993 and genistein R-2 = 0.9987). The detection limit for daidzein/genistein was 480/394 pg/mL (1.8/1.5 nM) and per column 2.4/1.9 pg. Isoflavones extracted from soybean products (farina, meat, milk) by the accelerated solvent extraction (ASE) procedure and isoflavones present in human urine were determined by the HPLC-ED method. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:101 / 111
页数:11
相关论文
共 58 条
[1]  
ADLERCREUTZ H, 1982, LANCET, V2, P1295
[2]   Phyto-oestrogens and Western diseases [J].
Adlercreutz, H ;
Mazur, W .
ANNALS OF MEDICINE, 1997, 29 (02) :95-120
[3]   Pressurized liquid extraction for the determination of polyphenols in apple [J].
Alonso-Salces, RM ;
Korta, E ;
Barranco, A ;
Berrueta, LA ;
Gallo, B ;
Vicente, F .
JOURNAL OF CHROMATOGRAPHY A, 2001, 933 (1-2) :37-43
[4]   ORIGIN OF LIGNANS IN MAMMALS AND IDENTIFICATION OF A PRECURSOR FROM PLANTS [J].
AXELSON, M ;
SJOVALL, J ;
GUSTAFSSON, BE ;
SETCHELL, KDR .
NATURE, 1982, 298 (5875) :659-660
[5]   Square-wave voltammetric determination of cefoperazone in a bacterial culture, pharmaceutical drug, milk, and urine [J].
Billová, S ;
Kizek, R ;
Jelen, F ;
Novotná, P .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2003, 377 (02) :362-369
[6]   STRESS RESPONSES IN ALFALFA (MEDICAGO-SATIVA L) .16. ANTIFUNGAL ACTIVITY OF MEDICARPIN AND ITS BIOSYNTHETIC PRECURSORS - IMPLICATIONS FOR THE GENETIC MANIPULATION OF STRESS METABOLITES [J].
BLOUNT, JW ;
DIXON, RA ;
PAIVA, NL .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1992, 41 (05) :333-349
[7]   APPLICATION HINTS FOR SAVITZKY-GOLAY DIGITAL SMOOTHING FILTERS [J].
BROMBA, MUA ;
ZIEGLER, H .
ANALYTICAL CHEMISTRY, 1981, 53 (11) :1583-1586
[8]   Recent extraction techniques for solid matrices-supercritical fluid extraction, pressurized fluid extraction and microwave-assisted extraction: their potential and pitfalls [J].
Camel, V .
ANALYST, 2001, 126 (07) :1182-1193
[9]   Determination of puerarin and daidzein in Puerariae radix and its medicinal preparations by micellar electrokinetic capillary chromatography with electrochemical detection [J].
Cao, YH ;
Lou, CG ;
Zhang, X ;
Chu, QC ;
Fang, YZ ;
Ye, JN .
ANALYTICA CHIMICA ACTA, 2002, 452 (01) :123-128
[10]  
Cazanove F, 1997, J CHROMATOGR B, V690, P203