Initial study of two-phase laminar flow extraction chip for sample preparation for gas chromatography

被引：44

作者：

Xiao, Hong

Liang, Dong

Liu, Guanchao

Guo, Min

Xing, Wanli

Cheng, Jing ^{[1
]}

机构：

[1] Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China

[2] Tsing Hua Univ, Sch Med, Med Syst Biol Res Ctr, Beijing 100084, Peoples R China

[3] Natl Engn Res Ctr Beijing Biochip Technol, Beijing 102206, Peoples R China

[4] Tsing Hua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China

[5] CapitalBio Corp, Beijing 102206, Peoples R China

来源：

LAB ON A CHIP | 2006年 / 6卷 / 08期

关键词：

D O I：

10.1039/b600374e

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sample preparation method for gas chromatography using a two-phase, laminar flow extraction PDMS/glass chip has been developed. A stable two-phase laminar interface was obtained by surface modification, and the organic extraction phase and the aqueous sample phase were separated effectively when the two-phase laminar flows exit the chip. Experiments were conducted on the chip to extract ephedrine from aqueous solution. Good reproducibility was obtained over the entire range of ephedrine concentration using the extraction chips (CV range 2.7%-4.5%). Effects of salt and solvent on extraction efficiency were studied.

引用

页码：1067 / 1072

页数：6

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