A folic acid-based functionalized surface for biosensor systems

被引：11

作者：

Bhalerao, Kaustubh D. ^{[1
]}

Lee, Stephen C.

Soboyejo, Winston O.

Soboyejo, Alfred B. O.

机构：

[1] Univ Illinois, Dept Agr & Biol Engn, Urbana, IL 61801 USA

[2] Ohio State Univ, Dept Biomed Engn, Columbus, OH 43210 USA

[3] Ohio State Univ, Dept Chem Engn, Columbus, OH 43210 USA

[4] Ohio State Univ, Davis Heart & Lung Inst, Columbus, OH 43210 USA

[5] Princeton Univ, Dept Mech & Aerosp Engn, Princeton, NJ 08544 USA

[6] Princeton Univ, Princeton Mat Inst, Princeton, NJ 08544 USA

[7] Ohio State Univ, Dept Food Agr & Biol Engn, Columbus, OH 43210 USA

[8] Ohio State Univ, Dept Aerosp Engn, Columbus, OH 43210 USA

[9] Ohio State Univ, John Glenn Inst Publ Policy & Publ Serv, Columbus, OH 43210 USA

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2007年 / 18卷 / 01期

关键词：

FOLATE; DELIVERY; CELLS; VECTORS; TISSUES; SILANE; BOND;

D O I：

10.1007/s10856-006-0657-x

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

biological system. This interface imparts a biologically relevant function to the device and provides a measure of specificity towards the biological analyte of interest. This paper documents the choice of folic acid as the functional component of a cantilever sensor to recognize nasopharyngeal (KB) cancer cells. A conjugation chemistry protocol has been outlined to deploy folic acid onto a titanium-coated sensor surface using a silane linker. The presence and biological activity of the sensor was verified by means of an immmunospecific (ELISA) procedure. The overall performance of the folic acid-based cantilever sensor was measured using cancerous KB cell-binding experiments.

引用

页码：3 / 8

页数：6

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