A cholesterol-rich diet causes a greater hypercholesterolemic response in pregnant than in nonpregnant rats and does not modify fetal lipoprotein profile

To determine whether pregnancy modifies the hyperlipidemic response to a cholesterol-rich diet, pregnant and virgin rats were fed a semisynthetic diet supplemented (CRD) or not (CD) with 2% cholesterol and 1% cholic acid and studied at d 20 of treatment and/or gestation. Plasma triglycerides, free fatty acids and glycerol and liver triglycerides were greater in pregnant than in virgin rats fed CRD, The increase in both plasma and liver cholesterol caused by CRD did not differ in the two groups. In rats fed CD, hepatic lipase activity in liver was lower in pregnant than in virgin rats, while in those fed CRD, virgin rats had lower activity than those fed CD, Plasma VLDL-triglycerides were higher and LDL-triglycerides lower in pregnant than in virgin rats fed CD, Among those fed CRD, pregnant rats had a higher triglyceride concentration in VLDL and HDL than virgin rats. Cholesterol concentration was higher in VLDL and IDL and lower in HDL in both groups fed CRD than in those fed CD, while cholesterol level in LDL was higher only in pregnant rats fed CRD than in those fed CD. Whereas placental cholesterol concentration was higher in pregnant rats fed CRD than CD, maternal CRD intake did not modify fetal plasma lipoprotein concentrations, fetal body weight or litter size, indicating a lack of cholesterol transfer by the rat placenta. Results therefore show a greater responsiveness to CRD in pregnant than in virgin rats, and we propose that CRD promotes greater liver VLDL-production and lower LDL removal in pregnant than in virgin rats.