Purification, characterization and structural analysis of an abundant β-1,3-glucanase from banana fruit

被引：42

作者：

Peumans, WJ

Barre, A

Derycke, V

Rougé, P

Zhang, WL

May, GD

Delcour, JA

Van Leuven, F

Van Damme, EJM

机构：

[1] Katholieke Univ Leuven, Lab Phytopathol & Plant Protect, B-3001 Louvain, Belgium

[2] Inst Pharmacol & Biol Struct, UPR CNRS 9062, Toulouse, France

[3] Katholieke Univ Leuven, Food Chem Lab, B-3001 Louvain, Belgium

[4] Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK 73402 USA

[5] Katholieke Univ Leuven, Ctr Human Genet, B-3001 Louvain, Belgium

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 2000年 / 267卷 / 04期

关键词：

beta-1,3-glucanase; Musa; PR protein;

D O I：

10.1046/j.1432-1327.2000.01117.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An abundant, catalytically active beta-1,3-endoglucanase (EC 3.2.1.39) has been isolated from the pulp of ripe bananas. Biochemical analysis of the purified protein, molecular modelling, and molecular cloning of the corresponding gene indicate that this banana enzyme closely resembles previously characterized plant beta-glucanases with respect to its amino-acid sequence, structure and biological activity. The results described in this paper demonstrate both the occurrence of an abundant active beta-1,3-endoglucanases in fruits and also readdress the question of the possible involvement of these enzymes in the ripening and/or softening process.

引用

页码：1188 / 1195

页数：8

共 31 条

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