Evaluation of a real-time PCR assay using the LightCycler System for detection of parvovirus B19 DNA

被引：6

作者：

Buller, RS ^{[1
]}

Storch, G ^{[1
]}

机构：

[1] Washington Univ, St Louis Childrens Hosp, Dept Pediat, Sch Med, St Louis, MO 63110 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 07期

关键词：

D O I：

10.1128/JCM.42.7.3326-3328.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We evaluated the artus ReatArt Parvovirus B19 LC PCR reagent (artus biotech USA, San Francisco, Calif.) for real-time PCR detection of parvovirus B19 DNA by retesting 71 specimens previously submitted to our laboratory. The artus assay, which produces a quantitative result and provides an internal PCR control, appeared to be slightly more sensitive than our conventional qualitative PCR assay.

引用

页码：3326 / 3328

页数：3

共 11 条

[1] HUMAN PARVOVIRUS INFECTION IN PREGNANCY AND HYDROPS-FETALIS [J].

ANAND, A ;

GRAY, ES ;

BROWN, T ;

CLEWLEY, JP ;

COHEN, BJ .

NEW ENGLAND JOURNAL OF MEDICINE, 1987, 316 (04) :183-186

[2]

ANDERSON MJ, 1983, LANCET, V1, P1378

[3] EXPERIMENTAL PARVOVIRAL INFECTION IN HUMANS [J].

ANDERSON, MJ ;

HIGGINS, PG ;

DAVIS, LR ;

WILLMAN, JS ;

JONES, SE ;

KIDD, IM ;

PATTISON, JR ;

TYRRELL, DAJ .

JOURNAL OF INFECTIOUS DISEASES, 1985, 152 (02) :257-265

[4] Neurological manifestations of human parvovirus B19 infection [J].

Barah, F ;

Vallely, PJ ;

Cleator, GM ;

Kerr, JR .

REVIEWS IN MEDICAL VIROLOGY, 2003, 13 (03) :185-199

[5]

BROWN T, 1984, LANCET, V2, P1033

[6] DETECTION OF HUMAN PARVOVIRUS B19 DNA BY USING THE POLYMERASE CHAIN-REACTION [J].

KOCH, WC ;

ADLER, SP .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (01) :65-69

[7] PURE RED-CELL APLASIA OF 10 YEARS DURATION DUE TO PERSISTENT PARVOVIRUS-B19 INFECTION AND ITS CURE WITH IMMUNOGLOBULIN THERAPY [J].

KURTZMAN, G ;

FRICKHOFEN, N ;

KIMBALL, J ;

JENKINS, DW ;

NIENHUIS, AW ;

YOUNG, NS .

NEW ENGLAND JOURNAL OF MEDICINE, 1989, 321 (08) :519-523

[8] CHRONIC BONE-MARROW FAILURE DUE TO PERSISTENT B19-PARVOVIRUS INFECTION [J].

KURTZMAN, GJ ;

OZAWA, K ;

COHEN, B ;

HANSON, G ;

OSEAS, R ;

YOUNG, NS .

NEW ENGLAND JOURNAL OF MEDICINE, 1987, 317 (05) :287-294

[9]

PATTISON JR, 1981, LANCET, V1, P664

[10] Establishment of the first World Health Organization International Standard for human parvovirus B19 DNA nucleic acid amplification techniques [J].

Saldanha, J ;

Lelie, N ;

Yu, MW ;

Heath, A .

VOX SANGUINIS, 2002, 82 (01) :24-31

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