Subventricular zone cells remain stable in vitro after brain injury

被引：20

作者：

Dizon, M. L. V. ^{[1
]}

Shin, L. ^{[1
]}

Sundholm-Peters, N. L. ^{[1
]}

Kang, E. ^{[1
]}

Szele, F. G. ^{[1
]}

机构：

[1] Northwestern Univ, Childrens Mem Hosp, Feinberg Sch Med, Dept Pediat,CMRC Neurobiol Program, Chicago, IL 60614 USA

来源：

NEUROSCIENCE | 2006年 / 142卷 / 03期

关键词：

migration; neurogenesis; traumatic brain injury; subependymal zone; fate; mouse;

D O I：

10.1016/j.neuroscience.2006.06.050

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Subventricular zone (SVZ) cells emigrate toward brain injury but relatively few survive. Thus, if they are to be used for repair, ex vivo expansion and autologous transplantation of SVZ cells may be necessary. Since it is unclear how brain injury alters SVZ cell culture, we studied neurosphere formation, differentiation, and migration, after cortical lesions. The number of neurosphere forming cells from lesioned mice was comparable to controls. Also, the proportion of astrocytes and neurons generated in vitro remained unchanged after cortical lesions. Cell emigration from neurospheres was characterized by increased cell-cell contact after injury in adults and neonates. However, neither molecules implicated in SVZ migration nor the extent of migration changed after injury. Thus, neurospheres can be successfully cultured after extensive brain damage, and they are remarkably stable in vitro, suggesting suitability for ex vivo expansion and autologous transplantation. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.

引用

页码：717 / 725

页数：9

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