Molecular analysis of microscopic ezrin dynamics by two-photon FRAP

被引：89

作者：

Coscoy, S

Waharte, F

Gautreau, A

Martin, M

Louvard, D

Mangeat, P

Arpin, M

Amblard, F

机构：

[1] CNRS, Inst Curie, Lab Physicochim, Unite Mixte Rech 168, F-75248 Paris 05, France

[2] CNRS, Inst Curie, Unite Mixte Rech 144, F-75248 Paris, France

[3] Univ Montpellier 2, CNRS, Unite Mixte Rech 5539, F-34095 Montpellier 5, France

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2002年 / 99卷 / 20期

关键词：

D O I：

10.1073/pnas.192084599

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ezrin plays a key role in coupling signal transduction to cortical cell organization. This actin-membrane linker undergoes a series of conformational changes that modulate its interactions with various partners and its localization in membrane or cytosolic pools. Its mobility and exchange rates within and between these two pools were assessed by two-photon fluorescence recovery after photo-bleaching in epithelial cell microvilli. Analysis of ezrin mutants with an altered actin-binding site revealed three ezrin membrane states of different mobilities and exchange properties, reflecting sequential association with membrane components and F-actin in the context of a fast overall turnover.

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收藏

页码：12813 / 12818

页数：6

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