Staphylococcus aureus nuclease is a useful secretion reporter for mycobacteria

被引：21

作者：

Downing, KJ

McAdam, RA

Mizrahi, V

机构：

[1] S African Inst Med Res, Mol Biol Unit, ZA-2000 Johannesburg, South Africa

[2] Glaxo Wellcome Med Res Ctr, Immunopathol Unit, Stevenage, Herts, England

[3] Univ Witwatersrand, Sch Med, Dept Haematol, Johannesburg, South Africa

来源：

GENE | 1999年 / 239卷 / 02期

基金：

英国医学研究理事会;

关键词：

antigens; export; leader peptide; tuberculosis;

D O I：

10.1016/S0378-1119(99)00408-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 [遗传学]; 090102 [作物遗传育种];

摘要：

A secretion reporter system based on Staphylococcus aureus nuclease (nuc) was developed for use in mycobacteria. Fusion of secretion signals to the reporter cloned in a shuttle vector, pBPnuc1, resulted in halo formation around colonies of Mycobacterium smegmatis and Mycobacterium tuberculosis grown on DNase agar plates containing Methyl Green indicator dye. This in-situ detection system was used to identify secreted proteins by screening a pBPnuc1::H37Rv nuc gene fusion library in M. smegmatis. The clones identified in this screen all formed colony halos when present in M. tuberculosis grown on indicator media. The proteins corresponded to DesA2, a stearoyl-acyl carrier protein desaturase, PepA, a putative serine protease and the Apa antigen, which is the ATP-binding subunit of an ABC transport system. Of these proteins, only PepA and Apa contained recognizable leader peptides. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：293 / 299

页数：7

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