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Determinants for Escherichia coli RNA polymerase assembly within the beta subunit

被引：34
作者：
Wang, Y
Severinov, K
Loizos, N
Fenyo, D
Heyduk, E
Heyduk, T
Chait, BT
Darst, SA
机构：
[1] ROCKEFELLER UNIV, MOL BIOPHYS LAB, NEW YORK, NY 10021 USA
[2] ROCKEFELLER UNIV, LAB MASS SPECTROMETRY & GASEOUS ION CHEM, NEW YORK, NY 10021 USA
[3] ST LOUIS UNIV, SCH MED, DEPT BIOCHEM & MOL BIOL, ST LOUIS, MO 63104 USA
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1997年 / 270卷 / 05期
关键词：
E-coli RNA polymerase assembly; transcription; limited proteolysis; protein-protein footprinting;
D O I：
10.1006/jmbi.1997.1139
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We used binding assays and other approaches to identify fragments of the Escherichia coli RNAP beta subunit involved in the obligatory interaction with the alpha subunit to form the stable assembly intermediate alpha(2) beta as well as in the interaction to recruit the beta' subunit into the alpha(2) beta sub-assembly. We show that two regions of evolutionarily conserved sequence near the C terminus of beta (conserved regions H and I) are central to the assembly of RNAP and likely make subunit-subunit contacts with both alpha and beta'. (C) 1997 Academic Press Limited.
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页码：648 / 662
页数：15
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