Prolactin stimulates mitogen-activated protein kinase in human leiomyoma cells

被引：28

作者：

Nohara, A

Ohmichi, M

Koike, K

Jikihara, H

Kimura, A

Masuhara, K

Ikegami, H

Inoue, M

Miyake, A

Murata, Y

机构：

[1] OSAKA UNIV,SCH MED,DEPT OBSTET & GYNECOL,SUITA,OSAKA 565,JAPAN

[2] KANAZAWA UNIV,SCH MED,DEPT OBSTET & GYNECOL,KANAZAWA,ISHIKAWA 920,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 238卷 / 02期

关键词：

D O I：

10.1006/bbrc.1997.7322

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of prolactin (PRL) on proliferation of cultured human uterine leiomyoma-derived smooth muscle cells (SMC) and its mechanism of action were investigated. PRL stimulated DNA synthesis and the expression of PRL receptor was identified by ribonuclease protection assay. Moreover, the regulation of mitogen-activated protein (MAP) kinase by PRL in leiomyoma-derived SMC was investigated. PRL stimulated MAP kinase activity, as detected by P-32 incorporation into MAP-S, in a dose-dependent manner. PRL also rapidly stimulated MAP kinase phosphorylation as detected by in vivo phosphorylation using P-32 labeling and phosphotyrosine immunoblotting. These results suggest that PRL stimulates the proliferation of human leiomyoma cells via the MAP kinase cascade. (C) 1997 Academic Press.

引用

页码：473 / 477

页数：5

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