Recombinant nAG (a Salamander-Derived Protein) Decreases the Formation of Hypertrophic Scarring in the Rabbit Ear Model

被引：17

作者：

Al-Qattan, Mohammad M. ^{[1
,2
]}

Abd-Al Wahed, Mervat M. ^{[2
]}

Hawary, Khalid ^{[1
]}

Alhumidi, Ahmed A. ^{[3
]}

Shier, Medhat K. ^{[2
]}

机构：

[1] King Saud Univ, Dept Surg, Riyadh 11415, Saudi Arabia

[2] King Saud Univ, Coll Med, Res Ctr, Riyadh 11415, Saudi Arabia

[3] King Saud Univ, Dept Pathol, Riyadh, Saudi Arabia

来源：

BIOMED RESEARCH INTERNATIONAL | 2014年 / 2014卷

关键词：

SCARS;

D O I：

10.1155/2014/121098

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

nAG (newt-Anterrior Gradient) protein is the key mediator of regrowth of amputated limbs in salamanders. In a previous work in our lab, a new nAG gene (suitable for humans) was designed and cloned. The cloned vector was transfected into primary human fibroblasts. The expression of nAG in human primary fibroblasts was found to suppress collagen expression. The current study shows that local injection of recombinant nAG reduces scar hypertrophy in the rabbit ear model. This is associated with lower scar elevation index (SEI), lower levels of collagen I & III, higher levels of MMP1, and a higher degree of scar maturation in experimental wounds compared to controls.

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页数：5

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