Efficient stabilization of oligonucleotide duplexes through terpyridine metal complexes

被引：3

作者：

Freville, Fabrice ^{[1
]}

Pierre, Nathalie ^{[1
]}

Moreau, Serge ^{[1
]}

机构：

[1] Univ Bordeaux 2, INSERM, U386, F-33076 Bordeaux, France

来源：

CANADIAN JOURNAL OF CHEMISTRY | 2006年 / 84卷 / 06期

关键词：

oligonucleotides; metal complexes; terpyridine;

D O I：

10.1139/V06-080

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A large increase in the melting temperature of complementary oligonucleotides was obtained through the conjugation of terpyridine ligands at their nearby 5' or 3' ends and the addition of stoichiometric amounts of various transition metals. We describe here a short efficient synthetic route for the conjugation of terpyridine moieties and report new data on the stabilizing contribution of metal-assisted hybridization of oligonucleotides through UV-monitored melting temperature experiments.

引用

页码：854 / 858

页数：5

共 9 条

[1] The galvanization of biology: A growing appreciation for the roles of zinc [J].

Berg, JM ;

Shi, YG .

SCIENCE, 1996, 271 (5252) :1081-1085

[2] SYNTHESIS AND COORDINATION BEHAVIOR OF 6',6''-BIS(2-PYRIDYL)-2,2'-4,4''-2'',2'''-QUATERPYRIDINE - BACK-TO-BACK 2,2'-6',2''-TERPYRIDINE [J].

CONSTABLE, EC ;

WARD, MD .

JOURNAL OF THE CHEMICAL SOCIETY-DALTON TRANSACTIONS, 1990, (04) :1405-1409

[3] (R)-2,4-dihydroxybutyramide seco-pseudonucleosides:: New versatile homochiral synthons for synthesis of modified oligonucleotides [J].

Dioubankova, NN ;

Malakhov, AD ;

Stetsenko, DA ;

Korshun, VA ;

Gait, MJ .

ORGANIC LETTERS, 2002, 4 (26) :4607-4610

[4] Amino terminal Cu(II)- and Ni(II)-binding (ATCUN) motif of proteins and peptides: Metal binding, DNA cleavage, and other properties [J].

Harford, C ;

Sarkar, B .

ACCOUNTS OF CHEMICAL RESEARCH, 1997, 30 (03) :123-130

[5] A novel copper-mediated DNA base pair [J].

Meggers, E ;

Holland, PL ;

Tolman, WB ;

Romesberg, FE ;

Schultz, PG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (43) :10714-10715

[6] METALLOMICELLANOLS - INCORPORATION OF RUTHENIUM(II)-2,2'/6',2''-TERPYRIDINE TRIADS INTO CASCADE POLYMERS [J].

NEWKOME, GR ;

CARDULLO, F ;

CONSTABLE, EC ;

MOOREFIELD, CN ;

THOMPSON, AMWC .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1993, (11) :925-927

[7]

Pon Richard T., 1993, V20, P465

[8] 2,2′-bipyridine ligandoside:: A novel building block for modifying DNA with intra-duplex metal complexes [J].

Weizman, H ;

Tor, Y .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (14) :3375-3376

[9]

Zapata L, 2003, EUR J ORG CHEM, V2003, P1022

← 1 →