Drug-induced hypomethylation of a posttranscriptionally silenced transgene locus of tobacco leads to partial release of silencing

被引：41

作者：

Kovarík, A

Van Houdt, H

Holy, A

Depicker, A

机构：

[1] Acad Sci Czech Republ, Inst Biophys, CS-61265 Brno, Czech Republic

[2] State Univ Ghent VIB, Dept Mol & Plant Genet, B-9000 Ghent, Belgium

[3] Acad Sci Czech Republ, Inst Organ Chem & Biochem, CR-16610 Prague 6, Czech Republic

来源：

FEBS LETTERS | 2000年 / 467卷 / 01期

关键词：

DNA methylation; gene silencing; hypomethylation drug; T-DNA; tobacco;

D O I：

10.1016/S0014-5793(00)01077-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of DNA methylation upon posttranscriptional gene silencing (PTGS) has been investigated in transgenic tobacco Lines showing PTGS and methylation of the neomycin phosphotransferase II (nptII) reporter genes. Application of the hypomethylation drugs dihydroxypropyladenine or 5-azacytidine resulted in approximately 30% reduced methylation of cytosines located in a non-symmetrical context in the 3' untranslated region of the nptII transgenes. The hypomethylation was accompanied by up to 12-fold increase in NPTII protein levels, suggesting that methylation of non-symmetrical motifs may account for an increased degree of PTGS, Models for the possible role of DNA methylation in PTGS are discussed. (C) 2000 Federation of European Biochemical Societies.

引用

页码：47 / 51

页数：5

共 35 条

[1] REPRESSION OF GENES BY DNA METHYLATION DEPENDS ON CPG DENSITY AND PROMOTER STRENGTH - EVIDENCE FOR INVOLVEMENT OF A METHYL-CPG BINDING-PROTEIN [J].