Left-right asymmetric expression of lefty2 and nodal is induced by a signaling pathway that includes the transcription factor FAST2

被引：197

作者：

Saijoh, Y

Adachi, H

Sakuma, R

Yeo, CY

Yashiro, K

Watanabe, M

Hashiguchi, H

Mochida, K

Ohishi, S

Kawabata, M

Miyazono, K

Whitman, M

Hamada, H

机构：

[1] Osaka Univ, Inst Mol & Cellular Biol, Div Mol Biol, Suita, Osaka 5650871, Japan

[2] Japan Sci & Technol Corp, Core Res & Evolut Sci & Technol, Suita, Osaka 5650871, Japan

[3] Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA

来源：

MOLECULAR CELL | 2000年 / 5卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S1097-2765(00)80401-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The left-right (L-R) asymmetric expression of lefty2 and nodal is controlled by a left side-specific enhancer (ASE). The transcription factor FAST2, which can mediate signaling by TGF beta and activin, has now been identified as a protein that binds to a conserved sequence in ASE. These FAST2 binding sites were both essential and sufficient for L-R asymmetric gene expression. The Fast2 gene is bilaterally expressed when nodal and lefty2 are expressed on the left side. TGF beta and activin can activate the ASE activity in a FAST2-dependent manner, while Nodal can do so in the presence of an EGF-CFC protein. These results suggest that the asymmetric expression of leffy2 and nodal is induced by a left side-specific TGF beta-related factor, which is most likely Nodal itself.

引用

页码：35 / 47

页数：13

共 72 条

[1] Determination of left right asymmetric expression of nodal by a left side-specific enhancer with sequence similarity to a lefty-2 enhancer [J].