Micro-spectroscopy of chloroplasts in protoplasts from Arabidopsis thaliana under single- and multi-photon excitations

被引：6

作者：

Kao, FJ ^{[1
]}

Wang, YM

Chen, JC

Cheng, PC

Chen, RW

Lin, BL

机构：

[1] Natl Sun Yat Sen Univ, Ctr Neurosci, Kaohsiung 80424, Taiwan

[2] Natl Sun Yat Sen Univ, Dept Phys, Kaohsiung 80424, Taiwan

[3] SUNY Buffalo, Dept Elect Engn, Buffalo, NY 14260 USA

[4] Acad Sinica, Inst Mol Biol, Taipei 11529, Taiwan

来源：

JOURNAL OF LUMINESCENCE | 2002年 / 98卷 / 1-4期

关键词：

micro-spectroscopy; multi-photon excitation; photo-bleaching; chloroplasts;

D O I：

10.1016/S0022-2313(02)00258-2

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Ultrafast laser pulses are known to generate impacts on specimens in ways much different from cw illumination in the applications of confocal microscopy. In this study. protoplasts from Arabidopsis thaliana are employed as the samples to compare the effects induced by single- and multi-photon excitations under confocal imaging configuration, Through time-lapsed micro-spectroscopy we are able to characterize the differences in photo-bleaching and the evolution of fluorescence spectra of chloroplasts thus induced. These differences may be attributed to the disruption of chained reaction as a result of multi-photon excitation in photosynthesis. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：107 / 114

页数：8

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