Live Cell Linear Dichroism Imaging Reveals Extensive Membrane Ruffling within the Docking Structure of Natural Killer Cell Immune Synapses

被引：30

作者：

Benninger, Richard K. P. ^{[2
]}

Vanherberghen, Bruno ^{[1
]}

Young, Stephen ^{[3
]}

Taner, Sabrina B. ^{[3
]}

Culley, Fiona I. ^{[3
]}

Schnyder, Tim ^{[3
]}

Neil, Mark A. A. ^{[2
]}

Wuestner, Daniel ^{[4
]}

French, Paul M. W. ^{[2
]}

Davis, Daniel M. ^{[3
]}

Onfelt, Bjorn ^{[1
,5
]}

机构：

[1] Royal Inst Technol, Dept Appl Phys, Stockholm, Sweden

[2] Univ London Imperial Coll Sci Technol & Med, Dept Phys, London, England

[3] Univ London Imperial Coll Sci Technol & Med, Div Cell & Mol Biol, London, England

[4] Univ So Denmark, Dept Biochem & Mol Biol, Odense, Denmark

[5] Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden

来源：

BIOPHYSICAL JOURNAL | 2009年 / 96卷 / 02期

基金：

英国医学研究理事会; 英国工程与自然科学研究理事会; 瑞典研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

MICROTUBULE-ORGANIZING CENTER; FLUORESCENCE; POLARIZATION; ORIENTATION; ACTIVATION;

D O I：

10.1016/j.bpj.2008.10.005

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We have applied fluorescence imaging of two-photon linear dichroism to measure the subresolution organization of the cell membrane during formation of the activating (cytolytic) natural killer (NK) cell immune synapse (IS). This approach revealed that the NK cell plasma membrane is convoluted into ruffles at the periphery, but not in the center of a mature cytolytic NK cell IS. Time-lapse imaging showed that the membrane ruffles formed at the initial point of contact between NK cells and target cells and then spread radialy across the intercellular contact as the size of the IS increased, becoming absent from the center of the mature synapse. Understanding the role of such extensive membrane ruff ling in the assembly of cytolytic synapses is an intriguing new goal.

引用

页码：L13 / L15

页数：3

共 11 条

[1] Dynamic interaction of VCAM-1 and ICAM-1 with moesin and ezrin in a novel endothelial docking structure for adherent leukocytes [J].

Barreiro, O ;

Yáñez-Mó, M ;

Serrador, JM ;

Montoya, MC ;

Vicente-Manzanares, M ;

Tejedor, R ;

Furthmayr, H ;

Sánchez-Madrid, F .

JOURNAL OF CELL BIOLOGY, 2002, 157 (07) :1233-1245

[2] Fluorescence imaging of two-photon linear dichroism:: Cholesterol depletion disrupts molecular orientation in cell membranes [J].

Benninger, RKP ;

Önfelt, B ;

Neil, MAA ;

Davis, DM ;

French, PMW .

BIOPHYSICAL JOURNAL, 2005, 88 (01) :609-622

[3] CD28-stimulated ERK2 phosphorylation is required for polarization of the microtubule organizing center and granules in YTS NK cells [J].

Chen, Xi ;

Allan, David S. J. ;

Krzewski, Konrad ;

Ge, Baoxue ;

Kopcow, Hernan ;

Strominger, Jack L. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (27) :10346-10351

[4] The human natural killer cell immune synapse [J].

Davis, DM ;

Chiu, I ;

Fassett, M ;

Cohen, GB ;

Mandelboim, O ;

Strominger, JL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (26) :15062-15067

[5] The Immunological Synapse: A Molecular Machine Controlling T Cell Activation [J].

Grakoui, Arash ;

Bromley, Shannon K. ;

Sumen, Cenk ;

Davis, Mark M. ;

Shaw, Andrey S. ;

Allen, Paul M. ;

Dustin, Michael L. .

JOURNAL OF IMMUNOLOGY, 2015, 194 (09) :221-227

[6] Cis interactions of immunoreceptors with MHC and non-MHC ligands [J].

Held, Werner ;

Mariuzza, Roy A. .

NATURE REVIEWS IMMUNOLOGY, 2008, 8 (04) :269-278

[7] POLARIZATION OF THE GOLGI-APPARATUS AND THE MICROTUBULE-ORGANIZING CENTER WITHIN CLONED NATURAL-KILLER CELLS BOUND TO THEIR TARGETS [J].

KUPFER, A ;

DENNERT, G ;

SINGER, SJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (23) :7224-7228

[8] Three-dimensional segregation of supramolecular activation clusters in T cells [J].

Monks, CRF ;

Freiberg, BA ;

Kupfer, H ;

Sciaky, N ;

Kupfer, A .

NATURE, 1998, 395 (6697) :82-86

[9] Cell membrane orientation visualized by polarized total internal reflection fluorescence [J].

Sund, SE ;

Swanson, JA ;

Axelrod, D .

BIOPHYSICAL JOURNAL, 1999, 77 (04) :2266-2283

[10] Th1 and Th2 cells form morphologically distinct immunological synapses [J].

Thauland, Timothy J. ;

Koguchi, Yoshinobu ;

Wetzel, Scott A. ;

Dustin, Michael L. ;

Parker, David C. .

JOURNAL OF IMMUNOLOGY, 2008, 181 (01) :393-399

← 1 2 →