Optimization of culture conditions for production of yeast biomass using bamboo wastewater by response surface methodology

被引：96

作者：

Li, Xin ^{[1
]}

Jia Ouyang ^{[1
]}

Xu, Yong ^{[1
]}

Chen, Mu ^{[1
]}

Song, Xiangyang ^{[1
]}

Yong, Qiang ^{[1
]}

Yu, Shiyuan ^{[1
]}

机构：

[1] Nanjing Forestry Univ, Minist Educ, Key Lab Forest Genet & Biotechnol, Nanjing 210037, Jiangsu Prov, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2009年 / 100卷 / 14期

关键词：

Bamboo wastewater; Candida utilis; Plackett-Burman design; Response surface methodology; SINGLE-CELL PROTEIN; CANDIDA-UTILIS; RICE POLISHINGS; ACID; MOLASSES;

D O I：

10.1016/j.biortech.2009.03.001

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Response surface methodology (RSM) was applied to optimize culture conditions for the growth of Candida utilis with bamboo wastewater. A significant influence of initial pH, fermentation time and yeast extract on biomass of C utilis was evaluated by Plackett-Burman design (PBD). These factors were further optimized using a central composite design (CCD) and RSM. A combination of initial pH 6.1, fermentation time 69 h and yeast extract 1.17 g/L was optimum for maximum biomass of C. utilis. A 1.7-fold enhancement of biomass of C utilis was gained after optimization in shake-flask cultivation. The biomass of C. utilis reached 19.17 g/L in 3 L fermentor. (C) 2009 Elsevier Ltd. All rights reserved.

引用

收藏

页码：3613 / 3617

页数：5

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