Quantum Dots As Ultrasensitive Nanoactuators and Sensors of Amyloid Aggregation in Live Cells

被引：64

作者：

Julia Roberti, M. ^{[1
,2
,3
,4
]}

Morgan, Marcos ^{[5
]}

Menendez, Guillermo ^{[1
]}

Pietrasanta, Lia I. ^{[5
]}

Jovin, Thomas M. ^{[2
,3
,4
]}

Jares-Erijman, Elizabeth A. ^{[1
]}

机构：

[1] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Organ, CIHIDECAR,CONICET, RA-1428 Buenos Aires, DF, Argentina

[2] Max Planck Inst Biophys Chem, Lab Cellular Dynam, D-37077 Gottingen, Germany

[3] DFG Forschungszentrum, CMPB Mol Physiol Brain, Gottingen, Germany

[4] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Lab Max Planck Dinam Celular, Buenos Aires, DF, Argentina

[5] Univ Buenos Aires, Fac Ciencias Exactas & Nat, CONICET, Ctr Microscopias Avanzadas, Buenos Aires, DF, Argentina

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 23期

关键词：

KINETICS; PROBES;

D O I：

10.1021/ja900225w

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Quantum dots multifunctionalized with the amyloid protein (x-synuclein act at nanomolar concentrations as very potent inducers of the aggregation of micromolar-millimolar bulk concentrations of the protein in vitro and in cells. Fibrillation in live cells, a process diagnostic of Parkinson's disease, is accelerated up to 15-fold with only similar to 100 nanoparticles. The combination with a tetracysteine-tagged form of alpha-synuclein specific for fluorogenic biarsenicals constitutes a very sensitive system for studying pathological amyloid formation in cells.

引用

收藏

页码：8102 / 8107

页数：6

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