Regulated but not constitutive human respiratory syncytial virus (HRSV) P protein phosphorylation is essential for oligomerization

被引：27

作者：

Asenjo, A ^{[1
]}

Villanueva, N ^{[1
]}

机构：

[1] Inst Salud Carlos III, Ctr Nacl Microbiol, Madrid 28220, Spain

来源：

FEBS LETTERS | 2000年 / 467卷 / 2-3期

关键词：

human respiratory syncytial virus; P protein; phosphorylation; oligomerization;

D O I：

10.1016/S0014-5793(00)01171-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Purified human respiratory syncytial virus (HRSV) P phosphoprotein from transfected HEp-2 cells is able to oligomerize forming tetramers, The bulk of constitutive P protein phosphorylation (99.8%) (serine residues 116, 117, 119, 232 and 237) can be removed without affecting protein oligomerization, However, dephosphorylated P protein, produced in bacteria, is unable to oligomerize. This difference can be explained by a transient P protein phosphorylation, detected in HEp-2 cells, that could be essential for P protein oligomerization, (C) 2000 Federation of European Biochemical Societies.

引用

页码：279 / 284

页数：6

共 20 条

[1] 2-5A-DNA conjugate inhibition of respiratory syncytial virus replication: effects of oligonucleotide structure modifications and RNA target site selection [J].

Barnard, DL ;

Sidwell, RW ;

Xiao, W ;

Player, MR ;

Adah, SA ;

Torrence, PF .

ANTIVIRAL RESEARCH, 1999, 41 (03) :119-134

[2] INHIBITORY EFFECT OF A MARINE-SPONGE TOXIN, OKADAIC ACID, ON PROTEIN PHOSPHATASES - SPECIFICITY AND KINETICS [J].

BIALOJAN, C ;

TAKAI, A .

BIOCHEMICAL JOURNAL, 1988, 256 (01) :283-290

[3] Dissection of individual functions of the Sendai virus phosphoprotein in transcription [J].

Bowman, MC ;

Smallwood, S ;

Moyer, SA .

JOURNAL OF VIROLOGY, 1999, 73 (08) :6474-6483

[4]

COLLINS PL, 1995, VIROLOGY, P1313

[5] A role for the Sendai virus P protein trimer in RNA synthesis [J].

Curran, J .

JOURNAL OF VIROLOGY, 1998, 72 (05) :4274-4280

[6] Reexamination of the sendai virus P protein domains required for RNA synthesis: A possible supplemental role for the P protein [J].

Curran, J .

VIROLOGY, 1996, 221 (01) :130-140

[7] PARAMYXOVIRUS PHOSPHOPROTEINS FORM HOMOTRIMERS AS DETERMINED BY AN EPITOPE DILUTION ASSAY, VIA PREDICTED COILED COILS [J].

CURRAN, J ;

BOECK, R ;

LINMARQ, N ;

LUPAS, A ;

KOLAKOFSKY, D .

VIROLOGY, 1995, 214 (01) :139-149

[8]

DELACAMPA AG, 1988, J BIOL CHEM, V263, P14696

[9] Site-specific phosphorylation of the human immunodeficiency virus type-1 Rev protein accelerates formation of an efficient RNA-binding conformation [J].

Fouts, DE ;

True, HL ;

Cengel, KA ;

Celander, DW .

BIOCHEMISTRY, 1997, 36 (43) :13256-13262

[10] MULTIMERIZATION AND TRANSCRIPTIONAL ACTIVATION OF THE PHOSPHOPROTEIN (P) OF VESICULAR STOMATITIS-VIRUS BY CASEIN KINASE-II [J].

GAO, Y ;

LENARD, J .

EMBO JOURNAL, 1995, 14 (06) :1240-1247

← 1 2 →