Maize glutathione-dependent formaldehyde dehydrogenase cDNA: a novel plant gene of detoxification

被引:36
作者
Fliegmann, J [1 ]
Sandermann, H [1 ]
机构
[1] GSF FORSCHUNGSZENTRUM UMWELT & GESUNDHEIT,INST BIOCHEM PFLANZENPATHOL,D-85764 OBERSCHLEISSHEIM,GERMANY
关键词
alcohol dehydrogenase; enzyme evolution; formaldehyde detoxification; glutathione-dependent formaldehyde dehydrogenase; heterologous expression;
D O I
10.1023/A:1005872222490
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have previously shown that intact plants and cultured plant cells can metabolize and detoxify formaldehyde through the action of a glutathione-dependent formaldehyde dehydrogenase (FDH), followed by C-1 metabolism of the initial metabolite (formic acid). The cloning and heterologous expression of a cDNA for the glutathione-dependent formaldehyde dehydrogenase from Zea mays L. is now described. The functional expression of the maize cDNA in Escherichia coli proved that the cloned enzyme catalyses the NAD(+)-and glutathione (GSH)-dependent oxidation of formaldehyde. The deduced amino acid sequence of 41 kDa was on average 65% identical with class III alcohol dehydrogenases from animals and less than 60% identical with conventional plant alcohol dehydrogenases (ADH) utilizing ethanol. Genomic analysis suggested the existence of a single gene for this cDNA. Phylogenetic analysis supports the convergent evolution of ethanol-consuming ADHs in animals and plants from formaldehyde-detoxifying ancestors. The high structural conservation of present-day glutathione-dependent FDH in microorganisms, plants and animals is consistent with a universal importance of these detoxifying enzymes.
引用
收藏
页码:843 / 854
页数:12
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