A comparison of DNA extraction methods for food analysis

被引：102

作者：

Di Pinto, Angela ^{[1
]}

Forte, VitoTony

Guastadisegni, Maria Corsignano

Martino, Carmela

Schena, Francesco Paolo

Tantillo, Giuseppina

机构：

[1] Univ Bari, Dipartimento Sanita & Benessere Anim, Fac Med Vet, I-70010 Bari, Italy

[2] Apuliabiotech SCRL, I-70010 Bari, Italy

来源：

FOOD CONTROL | 2007年 / 18卷 / 01期

关键词：

food; DNA extraction; DNA purification; PCR;

D O I：

10.1016/j.foodcont.2005.08.011

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

In this paper, two DNA extraction and purification procedures for food analysis, Wizard (R) Magnetic DNA Purification for Food (Promega) and DNeasy (R) 'Tissue Kit (QIAGEN), have been compared concerning extraction efficiency, DNA purity and DNA suitability for amplification. The comparison of two extraction methods, in this study, has highlighted the efficiency of the Wizard (R) Magnetic DNA Purification approach for vegetable matrices while the revised DNeasy (R) Tissue Kit for complex and processed matrices. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：76 / 80

页数：5

共 16 条

[1] Comparison of seven RNA extraction methods on stool and shellfish samples prior to hepatitis A virus amplification
Arnal, C
Ferré-Aubineau, V
Besse, B
Mignotte, B
Schwartzbrod, L
Billaudel, S
[J]. JOURNAL OF VIROLOGICAL METHODS, 1999, 77 (01) : 17 - 26
[2] DETECTION OF ENTERIC VIRUSES IN OYSTERS BY USING THE POLYMERASE CHAIN-REACTION
ATMAR, RL
METCALF, TG
NEILL, FH
ESTES, MK
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (02) : 631 - 635
[3] Identification of cow's milk in "buffalo" cheese by duplex polymerase chain reaction
Bottero, MT
Civera, T
Anastasio, A
Turi, RM
Rosati, S
[J]. JOURNAL OF FOOD PROTECTION, 2002, 65 (02) : 362 - 366
[4] Calvo JH, 2001, J ANIM SCI, V79, P2108
[5] POLYETHYLENE-GLYCOL PRECIPITATION FOR RECOVERY OF PATHOGENIC VIRUSES, INCLUDING HEPATITIS-A VIRUS AND HUMAN ROTAVIRUS, FROM OYSTER, WATER, AND SEDIMENT SAMPLES
LEWIS, GD
METCALF, TG
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (08) : 1983 - 1988
[6] Validation of a method based on polymerase chain reaction for the detection of genetically modified organisms in various processed foodstuffs
Lipp, M
Bluth, A
Eyquem, F
Kruse, L
Schimmel, H
Van den Eede, G
Anklam, E
[J]. EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2001, 212 (04) : 497 - 504
[7] DNA-based methods for food authentication
Lockley, AK
Bardsley, RG
[J]. TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2000, 11 (02) : 67 - 77
[8] A quick and simple method for the identification of meat species and meat products by PCR assay
Matsunaga, T
Chikuni, K
Tanabe, R
Muroya, S
Shibata, K
Yamada, J
Shinmura, Y
[J]. MEAT SCIENCE, 1999, 51 (02) : 143 - 148
[9] Development and application of DNA analytical methods for the detection of GMOs in food
Meyer, R
[J]. FOOD CONTROL, 1999, 10 (06) : 391 - 399
[10] MEYR R, 1996, LEBENSMITTEL WISSENS, V29, P1

← 1 2 →