A BAC-based physical map of the major autosomes of Drosophila melanogaster

被引：103

作者：

Hoskins, RA ^{[1
]}

Nelson, CR

Berman, BP

Laverty, TR

George, RA

Ciesiolka, L

Naeemuddin, M

Arenson, AD

Durbin, J

David, RG

Tabor, PE

Bailey, MR

DeShazo, DR

Catanese, J

Mammoser, A

Osoegawa, K

de Jong, PJ

Celniker, SE

Gibbs, RA

Rubin, GM

Scherer, SE

机构：

[1] Univ Calif Berkeley, Lawrence Berkeley Lab, Berkeley Drosophila Genome Project, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley Drosophila Genome Project, Berkeley, CA 94720 USA

[3] Baylor Coll Med, Human Genome Sequencing Ctr, Houston, TX 77030 USA

[4] Roswell Pk Canc Inst, Dept Canc Genet, Buffalo, NY 14263 USA

来源：

SCIENCE | 2000年 / 287卷 / 5461期

关键词：

D O I：

10.1126/science.287.5461.2271

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We constructed a bacterial artificial chromosome (BAC)-based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.

引用

页码：2271 / 2274

页数：4

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