Overexpression of vascular endothelial growth factor induces cell transformation in cooperation with fibroblast growth factor 2

被引：19

作者：

Guerrin, M

Scotet, E

Malecaze, F

Houssaint, E

Plouet, J

机构：

[1] UPR 9006 CNRS, LAB BIOL MOL EUCARYOTE, F-31062 TOULOUSE, FRANCE

[2] INST BIOL, INSERM U211, F-44035 NANTES, FRANCE

来源：

ONCOGENE | 1997年 / 14卷 / 04期

关键词：

VEGF; FGF-2; retinal pigment epithelial cells; transformation;

D O I：

10.1038/sj.onc.1200846

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vascular endothelial growth factor (VEGF) is a family of homodimeric proteins produced from a single gene by alternative splicing of the VEGF transcript. VEGF induces irt vivo angiogenesis and vascular permeability. We have recently demonstrated that VEGF is an autocrine growth factor for retinal pigment epithelial (RPE) cells. To further understand the role of VEGF, we overexpressed VEGF in rat RPE cells. The transfected cells exhibited a growth advantage in vitro and an increased response to the mitogenic effect of fibroblasts growth factor-2 (FGF-2), and formed colonies in soft agar upon FGF-2 addition. Moreover, analysis of FGF-receptors evidenced a dramatic increase in FGFR-1 mRNA and protein level, supporting the hypothesis that this receptor mediates the transforming effect of FGF-2. These results reveal that the oncogenic role of VEGF is exerted through a cross regulation between VEGF and FGF signal transduction pathways.

引用

收藏

页码：463 / 471

页数：9

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