Germ-line transmission of transgenes in Xenopus laevis

被引：75

作者：

Marsh-Armstrong, N ^{[1
]}

Huang, HC ^{[1
]}

Berry, DL ^{[1
]}

Brown, DD ^{[1
]}

机构：

[1] Carnegie Inst Washington, Dept Embryol, Baltimore, MD 21210 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1999年 / 96卷 / 25期

关键词：

D O I：

10.1073/pnas.96.25.14389

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Adult Xenopus laevis frogs made transgenic by restriction enzyme-mediated integration were bred to test the feasibility of establishing lines of frogs that express transgenes. All of the 19 animals raised to sexual maturity generated progeny that expressed the transgene(s). The patterns and levels of expression of green fluorescent protein transgenes driven by a viral promoter, rat promoter, and four X. laevis promoters were all unaffected by passage through the germ line. These results demonstrate the ease of establishing transgenic lines in X. laevis.

引用

页码：14389 / 14393

页数：5

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