Applications of Proteomics in the Study of Inflammatory Bowel Diseases: Current Status and Future Directions with Available Technologies

Inflammatory bowel diseases (IBD) are chronic, heterogeneous and multifactorial intestinal inflammatory disorders. Major challenges in IBD research include identification of major pathogenic alterations of genes/proteins as well as effective biomarkers for early diagnosis, prop. and prediction of therapeutic response. Since proteins govern cellular structure and biological function, a wide selection of proteomic approaches enables effective characterization of IBD pathogenesis by investigation the dynamic nature of protein expression, cellular and subcellular distribution. posttranslational modifications. and interactions at both the cellular and subcellular levels. The aims of this review are to 1) highlight the Current status of proteomic Studies of IBD, and 2) introduce the available and emerging proteomic technologies that have potential applications, ill file study of IBD. These technologies include various mass spectrometry technologies. quantitative proteomics (2D-PAGE, ICAT, SILAC, iTRAQ). protein/antibody multi-epitope-ligand cartography. This review also presents information and methodologies. front sample selection and enrichment to protein identification, that are not only essential but also particularly relevant to IBD research. The potential future application of these technologies is expected to have a significant impact oil the discovery of novel biomarkers and key pathogenic factors for IBD.