Isolation and characterization of new microsatellites at the nm23-H1 and nm23-H2 gene loci and application for loss of heterozygosity (LOH) analysis

被引：10

作者：

Seifert, M

Theisinger, B

Engel, M

Seib, T

Seitz, G

Stolte, M

Hilgert, K

Welter, C

机构：

[1] UNIV SAARLAND,INST HUMANGENET,D-66421 HOMBURG,GERMANY

[2] KLINIKUM BAMBERG,INST PATHOL,D-96049 BAMBERG,GERMANY

[3] KLINIKUM BAYREUTH,INST PATHOL,D-95445 BAYREUTH,GERMANY

来源：

HUMAN GENETICS | 1997年 / 100卷 / 5-6期

关键词：

D O I：

10.1007/s004390050545

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The nm23-H1 gene has been suggested to be a metastasis suppressor gene. Studies about the events of loss of heterozygosity (LOH) at the nm23 locus and its correlation to metastasis are controversially discussed. To optimize detection of LOH at the nm23 locus, we screened two P1 clones for additional microsatellites. Tumor and normal DNA from 37 colorectal, 16 gastric, and 8 germ cancer patients were examined for LOH. We found two new CA repeats, one 5' to nm23-H1 and another 3' to nm23-H2. Using these nm23 locus-specific CA repeats and five other chromosome 17 loci (D17S1522, D17S1566, D17S855, D17S515, and TP53), allele loss was observed in 4/32 (12.5%) patients with colon cancer, 2/14 (14.3%) with gastric cancer, and 1/7 (14%) with germ cancer. No isolated LOH of the nm23 region was observed.

引用

页码：515 / 519

页数：5

共 23 条

[1] REDUCED EXPRESSION OF NM23 PROTEIN IS ASSOCIATED WITH ADVANCED TUMOR STAGE AND DISTANT METASTASES IN HUMAN COLORECTAL CARCINOMAS [J].