In vitro immunostimulatory properties of Abrus lectins derived peptides in tumor bearing mice

被引：37

作者：

Bhutia, Sujit K. ^{[1
]}

Mallick, Sanjaya K. ^{[1
]}

Maiti, Tapas K. ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India

来源：

PHYTOMEDICINE | 2009年 / 16卷 / 08期

关键词：

Abrus; Agglutinin; Abrin; Peptide; Dalton's lymphoma (DL); Immunostimulation; AGGLUTININ; MACROPHAGES; ACTIVATION; ANTITUMOR; CELLS; ASSAY;

D O I：

10.1016/j.phymed.2009.01.006

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

In vitro immunostimulatory effect of Abrus lectins derived peptide fractions (AGP and ABP) was investigated in DL bearing mice. Both AGP and ABP were found to activate splenocytes and induced production of cytokines like IL-2, IFN-gamma and TNF-alpha indicating a Th1 type of immune response. Analysis of in vitro treated splenocytes by flow cytometry revealed an increase in percentage of T and B cell with high expression of activation markers (CD25(+) and CD71(+)). At the same time, expression of co-stimulatory markers was significantly high compared to tumor control. The tumor associated macrophages were able to stimulate NO production, IL-1 secretion, increased phagocytosis and decreased expression of mannose receptor. It was also observed that NK cell was activated by AGP and ABP. These results suggest that both AGP and ABP act as immunostimulants in vitro in DL bearing mice. (C) 2009 Elsevier GmbH. All rights reserved.

引用

页码：776 / 782

页数：7

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