Protein separation using non-porous sorbents

This article overviews the development of non-porous sorbents having small particle diameters which have proven effective for rapid analysis and micropreparative separation of proteins by liquid chromatography. Much attention is given to the preparation and application of silica- and polystyrene-based non-porous packings for various chromatographic modes, especially affinity chromatography. Modeling works on the prediction and parameter estimation for the dynamics of protein adsorption using non-porous sorbents are reviewed and briefly described. To conclude this review, future prospects of the application of non-porous sorbents are also presented. (C) 1997 Elsevier Science B.V.