Cytokine expression in resorptive feline osteoclastic resorptive lesions

Feline osteoclastic resorptive lesions (FORL) of the teeth are common in cats, and lead to pain, destruction of the periodontal ligament, and tooth loss. The expression of interleukin (IL)- 1beta and IL-6 mRNA was higher in teeth with FORL than in normal teeth (P< 0(.)01 and P< 0(.)001, respectively), but no such differences were found between pathological and normal gingival tissue samples. There were no differences between teeth affected with FORL and normal teeth in respect of the expression of receptor activator of NFkappaB ligand (RANKL) mRNA or osteoprotegerin (OPG) mRNA. However, OPG mRNA expression was higher in gingival tissue associated with teeth affected with FORL than in normal gingival tissue (P< 0.05), whereas the reverse was true of RANKL mRNA expression (P< 0.05). OPG mRNA expression was significantly higher in teeth than in femoral and alveolar bone (P< 0(.)001). RANKL and OPG mRNAs were detected in all tissues examined. The data suggest that the elevated expression of IL-1beta and IL-6 mRNA plays a role in the mediation of osteoclast activity in advanced FORL. In contrast, OPG and RANKL do not appear to regulate osteoclasts in advanced disease. The results also suggest that OPG and RANKL mRNA play a role in mediating inflammatory responses in gingival cells, and that OPG has an inhibiting effect on tooth resorption. (C) 2002 Elsevier Science Ltd. All rights reserved.