Schizandra chinensis Alkaloids Inhibit Lipopolysaccharide-induced Inflammatory Responses in BV2 Microglial Cells

被引：13

作者：

Choi, Min Sik ^{[1
,2
]}

Kwon, Kyung Ja ^{[3
,4
]}

Jeon, Se Jin ^{[1
,2
]}

Go, Hyo Sang ^{[1
,2
]}

Kim, Ki Chan ^{[1
,2
]}

Ryu, Jae Ryun ^{[1
,2
]}

Lee, Jongmin ^{[3
,4
]}

Han, Seol-Heui ^{[3
,4
]}

Cheong, Jae Hoon ^{[5
]}

Ryu, Jong Hoon ^{[6
,7
]}

Bae, KiHwan

Shin, Chan Young ^{[3
,4
]}

Ko, Kwang Ho ^{[1
,2
]}

机构：

[1] Seoul Natl Univ, Coll Pharm, Dept Pharmacol, Seoul 151742, South Korea

[2] Seoul Natl Univ, Pharmaceut Sci Res Inst, Seoul 151742, South Korea

[3] Konkuk Univ, IBST, Ctr Geriatr Neurosci Res, Seoul 143701, South Korea

[4] Konkuk Univ, Sch Med, Seoul 143701, South Korea

[5] Sahmyook Univ, Dept Pharm, Seoul 139742, South Korea

[6] Kyung Hee Univ, Coll Pharm, Dept Oriental Pharmaceut Sci, Seoul 130701, South Korea

[7] Chungnam Natl Univ, Coll Pharm, Taejon 301721, South Korea

来源：

BIOMOLECULES & THERAPEUTICS | 2009年 / 17卷 / 01期

关键词：

Schizandra chinensis; Microglia; Activation; Nitric oxide (NO); Reactive oxygen species (ROS); Matrix metalloproteinase-9 (MMP-9); NF-KAPPA-B; NITRIC-OXIDE SYNTHASE; MATRIX METALLOPROTEINASES; GOMISIN-A; INDUCED APOPTOSIS; ACTIVATION; BRAIN; INDUCTION; MEDIATORS; PROTEIN;

D O I：

10.4062/biomolther.2009.17.1.47

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Schizandra chinensis (S. chinensis) exhibits a harmless, 'adaptogen-type' effect leading to improvements in mental performance and learning efficacy in brain. Activated microglia contributes to neuronal injury by releasing neurotoxic products, which make it important to regulate microglial activation to prevent further cytological as well as functional brain damage. However, the effect of S. chinensis on microglial activation has not been examined yet. We have investigated the effects of four compounds (Gomisin A, Gomisin N, Schizandrin and Schizandrol A) from S. chinensis on lipopolysaccharide (LPS)-induced microglial activation. In this study, BV2 microglial cells were activated with LIPS and the microglial activation was assessed by up-regulation of activation markers such as nitric oxide (NO), reactive oxygen species (ROS), and matrix metalloproteinase-9 (MMP-9). The results showed that all four compounds significantly reduced the intracellular level of ROS, the release of NO and MMP-9 as well as LPS-induced phosphorylation of ERK1/2. These results strongly suggested that S. chinensis may be useful to modulate inflammation-mediated brain damage by regulating microglial activation.

引用

页码：47 / 56

页数：10

共 53 条

[1]

Glial pathology but absence of apoptotic nigral neurons in long-standing Parkinson's disease [J].