Use of fluorescent lectin probes for analysis of footprints from Pseudomonas aeruginosa MDC on hydrophilic and hydrophobic glass substrata

被引：8

作者：

Bejarano, EM ^{[1
]}

Schneider, RP ^{[1
]}

机构：

[1] Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed, BR-05508900 Sao Paulo, Brazil

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 07期

关键词：

D O I：

10.1128/AEM.70.7.4356-4362.2004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microbial footprints of Pseudomonas aeruginosa MDC attached for 1 h to clean or silanized glass were analyzed with fluorescently labeled lectin probes. Footprint composition varied, depending on cell physiology and substratum surface chemistry. This suggests that substratum physicochemistry affected the structure of cell surfaces of adsorbed organisms.

引用

页码：4356 / 4362

页数：7

共 34 条

[1] Chemical and structural characterization of exopolymers produced by Pseudomonas sp NCIMB 2021 in continuous culture [J].

Beech, I ;

Hanjagsit, L ;

Kalaji, M ;

Neal, AL ;

Zinkevich, V .

MICROBIOLOGY-UK, 1999, 145 :1491-1497

[2] Structures of gram-negative cell walls and their derived membrane vesicles [J].

Beveridge, TJ .

JOURNAL OF BACTERIOLOGY, 1999, 181 (16) :4725-4733

[3] Investigation of lotic microbial aggregates by a combined technique of fluorescent in situ hybridization and lectin-binding-analysis [J].

Böckelmann, U ;

Manz, W ;

Neu, TR ;

Szewzyk, U .

JOURNAL OF MICROBIOLOGICAL METHODS, 2002, 49 (01) :75-87

[4] SPONTANEOUS RELEASE OF LIPOPOLYSACCHARIDE BY PSEUDOMONAS-AERUGINOSA [J].

CADIEUX, JE ;

KUZIO, J ;

MILAZZO, FH ;

KROPINSKI, AM .

JOURNAL OF BACTERIOLOGY, 1983, 155 (02) :817-825

[5] Exopolysaccharide production by mucoid and non-mucoid strains of Burkholderia cepacia [J].

Cérantola, S ;

Bounéry, JD ;

Segonds, C ;

Marty, N ;

Montrozier, H .

FEMS MICROBIOLOGY LETTERS, 2000, 185 (02) :243-246

[6] PARTIAL CHEMICAL AND PHYSICAL CHARACTERIZATION OF 2 EXTRACELLULAR POLYSACCHARIDES PRODUCED BY MARINE, PERIPHYTIC PSEUDOMONAS SP STRAIN NCMB-2021 [J].

CHRISTENSEN, BE ;

KJOSBAKKEN, J ;

SMIDSROD, O .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1985, 50 (04) :837-845

[7] The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formation [J].

Cramton, SE ;

Gerke, C ;

Schnell, NF ;

Nichols, WW ;

Götz, F .

INFECTION AND IMMUNITY, 1999, 67 (10) :5427-5433

[8] Rhamnolipid surfactant production affects biofilm architecture in Pseudomonas aeruginosa PAO1 [J].

Davey, ME ;

Caiazza, NC ;

O'Toole, GA .

JOURNAL OF BACTERIOLOGY, 2003, 185 (03) :1027-1036

[9] Initiation of biofilm formation by Pseudomonas aeruginosa 57RP correlates with emergence of hyperpiliated and highly adherent phenotypic variants deficient in swimming, swarming, and twitching motilities [J].

Déziel, E ;

Comeau, Y ;

Villemur, R .

JOURNAL OF BACTERIOLOGY, 2001, 183 (04) :1195-1204

[10] Identification of Tn10 insertions in the dsbA gene affecting Escherichia coli biofilm formation [J].

Genevaux, P ;

Bauda, P ;

DuBow, MS ;

Oudega, B .

FEMS MICROBIOLOGY LETTERS, 1999, 173 (02) :403-409

← 1 2 3 4 →