UvrD-dependent replication of rolling-circle plasmids in Escherichia coli

被引:78
作者
Bruand, C [1 ]
Ehrlich, SD [1 ]
机构
[1] INRA, Lab Genet Microbienne, F-78352 Jouy En Josas, France
关键词
D O I
10.1046/j.1365-2958.2000.01700.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Escherichia coli UvrD helicase (or helicase II) is known for its involvement in DNA repair. We report that UvrD is required for DNA replication of several different rolling-circle plasmids in E. coli, whereas its homologue, the Rep helicase, is not. Lack of UvrD helicase does not impair the first step of plasmid replication, nicking of the double-stranded origin by the plasmid initiator protein. However, replication proceeds no further without UvrD. Indeed, the nicked plasmid molecules accumulate to a high level in uvrD mutants. We conclude that UvrD is the replicative helicase of various rolling-circle plasmids. This is the first description of a direct implication of UvrD in DNA replication in vivo.
引用
收藏
页码:204 / 210
页数:7
相关论文
共 55 条
  • [1] Kinetic measurement of the step size of DNA unwinding by Escherichia coli UvrD helicase
    Ali, JA
    Lohman, TM
    [J]. SCIENCE, 1997, 275 (5298) : 377 - 380
  • [2] Role of E-coli DNA helicase II in a umuCD-dependent Wiegle reactivation
    Altshuler, ML
    [J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1996, 351 (01) : 3 - 7
  • [3] Arai K, 1981, Prog Nucleic Acid Res Mol Biol, V26, P9, DOI 10.1016/S0079-6603(08)60392-X
  • [4] uvrD mutations enhance tandem repeat deletion in the Escherichia coli chromosome via SOS induction of the RecF recombination pathway
    Bierne, H
    Seigneur, M
    Ehrlich, SD
    Michel, B
    [J]. MOLECULAR MICROBIOLOGY, 1997, 26 (03) : 557 - 567
  • [5] Characterisation of Bacillus stearothermophilus PcrA helicase:: evidence against an active rolling mechanism
    Bird, LE
    Brannigan, JA
    Subramanya, HS
    Wigley, DB
    [J]. NUCLEIC ACIDS RESEARCH, 1998, 26 (11) : 2686 - 2693
  • [6] REPLICATION ORIGINS OF SINGLE-STRANDED-DNA PLASMID PUB110
    BOE, L
    GROS, MF
    TERIELE, H
    EHRLICH, SD
    GRUSS, A
    [J]. JOURNAL OF BACTERIOLOGY, 1989, 171 (06) : 3366 - 3372
  • [7] DNA-INDUCED DIMERIZATION OF THE ESCHERICHIA-COLI REP HELICASE
    CHAO, KL
    LOHMAN, TM
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1991, 221 (04) : 1165 - 1181
  • [8] CLERGET M, 1991, NEW BIOL, V3, P780
  • [9] THE ESCHERICHIA-COLI REP MUTATION .10. CONSEQUENCES OF INCREASED AND DECREASED REP PROTEIN-LEVELS
    COLASANTI, J
    DENHARDT, DT
    [J]. MOLECULAR & GENERAL GENETICS, 1987, 209 (02): : 382 - 390
  • [10] Mismatch-, MutS-, MutL-, and helicase II-dependent unwinding from the single-strand break of an incised heteroduplex
    Dao, V
    Modrich, P
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (15) : 9202 - 9207