Transglycosylation reactions performed by glycosyl hydrolases from the marine anaspidean Aplysia fasciata

被引：19

作者：

Giordano, A ^{[1
]}

Andreotti, G ^{[1
]}

Mollo, E ^{[1
]}

Trincone, A ^{[1
]}

机构：

[1] CNR, Ist Chim Biomol, I-80078 Naples, Italy

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2004年 / 30卷 / 02期

关键词：

glycosidases; isomaltose; panose; marine organisms; Aplysia fasciata;

D O I：

10.1016/j.molcatb.2004.03.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The search for glycosyl hydrolytic activities from natural sources and the investigation of their transglycosylation potential can provide enzymes with new interesting catalytic activities for the synthesis of oligosaccharides. We focused our attention on the sea hare Aplysia fasciata Poiret, 1789, a common Mediterranean opistobranch mollusc, describing the results on the presence of different glycosyl hydrolases found in this organism. The activities of interest are present in the visceral mass and in the hepatopancreas extracts which were highly active with respect to those from other marine molluscs, namely an exo-alpha-D-glucosidase activity and a beta-galactosidase, were investigated. The former is able to produce panose in high percentage by transglycosylation from maltose, or alpha-D-glucosides of alpha-methyl mannopyranoside and 2-deoxyglucose using p-nitrophenyl alpha-D-glucopyranoside as donor. The beta-galactosidase from hepatopancreas produced in transgalactosylation reactions the beta-Gal-(1-4)-GlcNAc as the most abundant product and Gal-Xyl disaccharides of biological importance using p-nitrophenyl beta-D-galactopyranoside as donor and GlcNAc or benzyl alpha-xyloside as acceptors, respectively. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：51 / 59

页数：9

共 23 条

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