New anonymous nuclear DNA markers for the pearl oyster Pinctada margaritifera and other Pinctada species

被引：9

作者：

Arnaud-Haond, S ^{[1
]}

Boudry, P

Saulnier, D

Seaman, T

Bonhomme, F

Goyard, E

机构：

[1] IFREMER, COP, Lab Aquaculture Trop, BP 7004, F-98719 Taravao, Tahiti, France

[2] Serv Perliculture, Motu Uta, Tahiti, France

[3] Lab Genome Populat Interact, UMR 5000, Sete, France

来源：

MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 03期

关键词：

anonymous nuclear DNA; genetic resources; introns; length polymorphism; molecular markers; pearl oysters;

D O I：

10.1046/j.1471-8286.2002.00199.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The black-lipped oyster Pinctada margaritifera is highly exploited in French Polynesia where the pearl industry relies mostly on spat collection, and therefore on resources available in the wild. Little is known of these resources, and population genetic studies would be useful to improve management. We used two methods, direct amplification of length polymorphism (DALP) and exon primed intron crossing (EPIC) to develop five new nuclear markers presenting length polymorphism. Although these markers remained anonymous after using BLAST on GenBank, they are codominant and follow Hardy-Weinberg equilibrium. Tests on related species or subspecies of Pinctada gave encouraging results.

引用

页码：220 / 222

页数：3

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[No title captured]

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