A new method for identifying the amino acid attached to a particular RNA in the cell

被引：15

作者：

Suzuki, T

Ueda, T

Watanabe, K

机构：

[1] UNIV TOKYO,SCH ENGN,DEPT CHEM & BIOCHEM,BUNKYO KU,TOKYO 113,JAPAN

[2] TOKYO INST TECHNOL,FAC BIOSCI & BIOTECHNOL,DEPT BIOL SCI,MIDORI KU,YOKOHAMA,KANAGAWA 227,JAPAN

来源：

FEBS LETTERS | 1996年 / 381卷 / 03期

关键词：

aminoacyl-tRNA (in vivo); amino acid; hybridization; N-acetylation; Candida;

D O I：

10.1016/0014-5793(96)00107-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To investigate the function of tRNAs or any other aminoacylable RNAs in vivo, it is important to be able to estimate the amounts and species of aminoacylated RNAs in living cells. We have developed a method of analyzing amino acids attached to particular tRNAs obtained from cells. After the ester bond between the amino acid and the 3'-adenosine moiety of a specific aminoacyl-tRNA is stabilized by acetylation of the amino acid with [C-14]acetic anhydride, the aminoacyl-tRNA can be fished out with a solid-phase-attached DNA probe. The C-14-labeled acetylamino acid is then released from the thus purified acetyl-aminoacyl-tRNAs by alkaline treatment and detected by TLC analysis.

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页码：195 / 198

页数：4

相关论文

共 23 条

[1]

Florentz Catherine, 1995, P141

[2] A GENERAL PROCEDURE FOR ISOLATION OF SPECIFIC TRANSFER RIBONUCLEIC ACIDS [J].

GILLAM, I ;

BLEW, D ;

WARRINGTON, RC ;

VONTIGER.M ;

TENER, GM .

BIOCHEMISTRY, 1968, 7 (10) :3459-+

[3] BEHAVIOUR OF ACETYLPHENYLALANYL SOLUBLE RIBONUCLEIC ACID IN POLYPHENYLALANINE SYNTHESIS [J].

HAENNI, AL ;

CHAPEVILLE, F .

BIOCHIMICA ET BIOPHYSICA ACTA, 1966, 114 (01) :135-+

[4] INCORPORATION OF PHENYLLACTIC ACID IN TERMINAL POSITION DURING POLYPHENYLALANINE BIOSYNTHESIS [J].

HERVE, G ;

CHAPEVIL.F .

JOURNAL OF MOLECULAR BIOLOGY, 1965, 13 (03) :757-&

[5] A TRANSFER-RNA-LIKE STRUCTURE IS PRESENT IN 10SA RNA, A SMALL STABLE RNA FROM ESCHERICHIA-COLI [J].

KOMINE, Y ;

KITABATAKE, M ;

YOKOGAWA, T ;

NISHIKAWA, K ;

INOKUCHI, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (20) :9223-9227

[6] EFFECT OF THE HIGHER-ORDER STRUCTURE OF TRANSFER-RNAS ON THE STABILITY OF HYBRIDS WITH OLIGODEOXYRIBONUCLEOTIDES - SEPARATION OF TRANSFER-RNA BY AN EFFICIENT SOLUTION HYBRIDIZATION [J].

KUMAZAWA, Y ;

YOKOGAWA, T ;

TSURUI, H ;

MIURA, K ;

WATANABE, K .

NUCLEIC ACIDS RESEARCH, 1992, 20 (09) :2223-2232

[7] INVITRO SYNTHESIS OF SELENOCYSTEINYL-TRANSFER RNAUCA FROM SERYL-TRANSFER RNAUCA - INVOLVEMENT AND CHARACTERIZATION OF THE SELD GENE-PRODUCT [J].

LEINFELDER, W ;

FORCHHAMMER, K ;

VEPREK, B ;

ZEHELEIN, E ;

BOCK, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (02) :543-547

[8] GROWTH-RATE OF ESCHERICHIA-COLI [J].

MARR, AG .

MICROBIOLOGICAL REVIEWS, 1991, 55 (02) :316-333

[9] STARVATION IN-VIVO FOR AMINOACYL-TRANSFER-RNA INCREASES THE SPATIAL SEPARATION BETWEEN THE 2 RIBOSOMAL-SUBUNITS [J].

OFVERSTEDT, LG ;

ZHANG, K ;

TAPIO, S ;

SKOGLUND, U ;

ISAKSSON, LA .

CELL, 1994, 79 (04) :629-638

[10] NONUNIVERSAL DECODING OF THE LEUCINE CODON CUG IN SEVERAL CANDIDA SPECIES [J].

OHAMA, T ;

SUZUKI, T ;

MORI, M ;

OSAWA, S ;

UEDA, T ;

WATANABE, K ;

NAKASE, T .

NUCLEIC ACIDS RESEARCH, 1993, 21 (17) :4039-4045