Enzyme microarrays: On-chip determination of inhibition constants based on affinity-label defection of enzymatic activity

被引：30

作者：

Eppinger, J

Funeriu, DP

Miyake, M

Denizot, L

Miyake, J

机构：

[1] Tech Univ Munich, ForschungsDozentur Mol Katalyse Lehrstuhl Anorgan, D-85748 Garching, Germany

[2] Natl Inst Adv Ind Sci & Technol, Tissue Engn Res Ctr, Amagasaki, Hyogo 6610974, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 29期

关键词：

enzymes; fluorescent probes; high-throughput screening; inhibitors; microarrays; on-chip kinetics;

D O I：

10.1002/anie.200353623

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

High-throughput screening of libraries of potential inhibitors is possible by using an enzyme microarray method that combines low sample consumption with accuracy and speed. Information about enzyme inhibition can be obtained on-chip" by the addition of a fluorescently tagged affinity label to a microarray of functional enzymes (see picture)."

引用

收藏

页码：3806 / 3810

页数：5

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