Analysis of soil and bacterioplankton community DNA by melting profiles and reassociation kinetics

被引:26
作者
Ritz, K
Griffiths, BS
Torsvik, VL
Hendriksen, NB
机构
[1] UNIV BERGEN,DEPT MICROBIOL,N-5020 BERGEN,NORWAY
[2] NATL ENVIRONM RES INST,DEPT MARINE ECOL & MICROBIOL,DK-4000 ROSKILDE,DENMARK
关键词
bacterioplankton; community DNA; community structure; percent guanine+cytosine distribution; reassociation kinetics; soil community;
D O I
10.1111/j.1574-6968.1997.tb10322.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Whole-community DNA was extracted from two pasture soils, the cultivable and directly extractable bacterial subsets of one of the soils, and two aquatic bacterioplankton communities. The percent guanine+cytosine (%G+C) base distribution, derived using thermal denaturation profiles, and parameterised using a generalised logistic equation, varied markedly between the samples. Bacterioplankton communities carried a more even distribution of %G+C than the soil communities, which had higher median %G+C. The directly extracted soil bacterial fraction had the highest median %G+C. There was a distinct trend of increasing complexity, measured using reassociation kinetics, between cultivable, extractable and total soil community DNA. Bacterioplankton communities were of a similar order of complexity to the cultivable soil bacterial fraction.
引用
收藏
页码:151 / 156
页数:6
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