Capillary electrophoresis-mass spectrometry, an attractive tool for drug bioanalysis and biomarker discovery

被引:63
作者
Servais, Anne-Catherine [1 ]
Crommen, Jacques [1 ]
Fillet, Marianne [1 ]
机构
[1] Univ Liege, Inst Pharm, Dept Pharmaceut Analyt Chem, B-4000 Liege 1, Belgium
关键词
biological fluids; biomarker discovery; capillary electrophoresis-mass spectrometry; drugs;
D O I
10.1002/elps.200500934
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The coupling of CE with MS detection, a relatively recent hyphenated technique, has gained increasing respect in the field of bioanalytical applications over the past few years. The first part of this review presents CE-MS applications dealing with drug bioanalysis, including forensic analysis and metabolism studies. Practical considerations to achieve a robust and sensitive CE-MS coupling are also presented. It is indeed essential to strictly control some critical electrospray parameters, such as the sheath liquid composition and flow rate, the nebulizing gas pressure as well as the capillary outlet position. The second part of the review critically describes the applications of CE coupled on-line to MS for the identification of biomarkers in body fluids for diagnostic purposes. Since the sample preparation procedures strongly differ according to the intended use (drug bioanalysis or biomarker discovery), they are discussed separately, taking into account the particular properties of plasma and urine matrices.
引用
收藏
页码:2616 / 2629
页数:14
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